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Related Experiment Videos

Deep-etch views of clathrin assemblies.

J Heuser, T Kirchhausen

    Journal of Ultrastructure Research
    |July 1, 1985
    PubMed
    Summary
    This summary is machine-generated.

    New freeze-drying techniques reveal clathrin triskelions have unique features, modifying existing models of how these protein structures assemble into cellular coats. This study offers a revised clathrin assembly scheme.

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    Area of Science:

    • Structural Biology
    • Cell Biology
    • Biochemistry

    Background:

    • Clathrin assemblies form polygonal coats essential for intracellular trafficking.
    • Existing models, like the Crowther and Pearse scheme, describe clathrin triskelion assembly.
    • Understanding the precise mechanism of clathrin coat formation is crucial for cell biology.

    Purpose of the Study:

    • To investigate the detailed structure of clathrin trimers (triskelions) and their assembly into coats.
    • To re-evaluate and potentially modify existing models of clathrin coat assembly using advanced imaging.
    • To identify key structural features of clathrin trimers that dictate coat formation.

    Main Methods:

    • Adsorption of clathrin assemblies to mica.
    • A novel freeze-drying procedure for high-resolution 3-D imaging.

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  • Comparative analysis of individual triskelions and assembled polygonal networks.

    • Main Results:

    • Freeze-drying revealed terminal scroll-shaped hooks on trimer arms, influencing their length and assembly.
    • Extended trimer arms can span three sides of a polygonal facet, with excess length pointing inward.
    • Trimer arms exhibit specific bends, guiding their distribution in the final coat structure.
    • Assembled coat struts are uniform and cylindrical, differing from predictions of the Crowther and Pearse model.
    • A modified clathrin assembly scheme is proposed, suggesting initial slewed arm emergence but non-slewed final struts.

    Conclusions:

    • The study presents a revised model for clathrin coat assembly based on detailed structural observations.
    • The findings highlight the importance of triskelion arm morphology and flexibility in coat formation.
    • Further experiments are needed to validate the proposed assembly scheme and investigate assembly intermediates.