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Amplification of c-erbB-2 oncogene in human adenocarcinomas in vivo

J Yokota, T Yamamoto, K Toyoshima

    Lancet (London, England)
    |April 5, 1986
    PubMed

    Insights

    The c-erbB-2 gene is amplified in some adenocarcinomas, suggesting a role in their development. The epidermal growth factor (EGF) receptor gene (c-erbB-1) is amplified in a small number of squamous-cell carcinomas.

    Area of Science:

    • Molecular Biology
    • Oncology
    • Genetics

    Background:

    • The human genome contains two genes related to the viral erbB gene: c-erbB-1 (epidermal growth factor receptor) and c-erbB-2.
    • c-erbB-2 encodes a receptor-like protein distinct from the EGF receptor.

    Purpose of the Study:

    • To investigate the amplification of c-erbB-2 and c-erbB-1/EGF-receptor genes in human malignant tumors.
    • To determine the potential role of these amplified genes in specific cancer types.

    Main Methods:

    • DNA hybridization analysis was performed on 101 fresh human malignant tumors.
    • Tumors were analyzed for gene amplification of c-erbB-2 and c-erbB-1/EGF-receptor.

    Main Results:

    • The c-erbB-2 gene was amplified in 5 out of 63 adenocarcinomas.
    • No amplification of c-erbB-2 was found in 38 other tumor types.
    • The c-erbB-1/EGF-receptor gene was amplified in 1 out of 8 squamous-cell carcinomas.

    Conclusions:

    • Amplification of the c-erbB-2 gene may play a role in the evolution of adenocarcinomas.
    • The EGF receptor gene may be involved in the development of some squamous-cell carcinomas.

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