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Atomic Force Microscopy01:08

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Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
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Can photoacoustic imaging quantify surface-localized J-aggregating nanoparticles?

Liang Lim1, Robert Mastragostino1, Kenneth Ng2

  • 1University Health Network, Princess Margaret Cancer Centre, Toronto, Ontario, Canada.

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|July 14, 2017
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Photoacoustic (PA) imaging can quantify surface nanoparticle concentration. This study demonstrates a wide linear range for J-aggregating porphysome (JNP) detection, crucial for topical nanoparticle applications in diagnostics.

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Area of Science:

  • Biomedical Optics
  • Photoacoustic Imaging
  • Nanotechnology

Background:

  • Surface-localized nanoparticles are crucial for targeted drug delivery and diagnostics.
  • Accurate quantification of these nanoparticles is essential for therapeutic efficacy and monitoring.
  • Photoacoustic imaging offers high contrast and resolution for deep tissue visualization.

Purpose of the Study:

  • To assess the feasibility of photoacoustic (PA) imaging for quantifying surface-localized nanoparticles.
  • To determine the linear range and accuracy of PA signal intensity in relation to nanoparticle concentration.
  • To evaluate the impact of tissue optical properties on PA quantification of nanoparticles.

Main Methods:

  • Utilized tissue-mimicking phantoms with varying concentrations of J-aggregating porphysomes (JNP).
  • Employed a commercial PA instrument at 815 nm and a 40 MHz linear-array transducer.
  • Analyzed the relationship between PA signal intensity and JNP areal concentration under different optical conditions.

Main Results:

  • Established a reproducible linear range of PA signal intensity from ~17 fmol/mm2 to 11 pmol/mm2 (approximately 3 orders of magnitude) with ±34% error.
  • Demonstrated that for scattering-dominated tissues (albedo >0.8) and concentrations above ~330 fmol/mm2, PA signal is independent of tissue optical properties.
  • Identified the necessity of measuring underlying tissue optical properties for accurate quantification in other conditions.

Conclusions:

  • Photoacoustic imaging is a feasible method for quantifying surface-localized nanoparticles.
  • The study defines the operational range and limitations for accurate quantification in various tissue optical environments.
  • Findings support the application of PA imaging for monitoring topical nanoparticle delivery in areas like endoscopic diagnosis.