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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

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Overexpression miR-211-5p hinders the proliferation, migration, and invasion of thyroid tumor cells by downregulating

Lei Wang1, Yan-Feng Shen2, Zhi-Min Shi1

  • 1Department of Pathology, Medical College of Hebei University of Engineering, Handan, Hebei Province, China.

Journal of Clinical Laboratory Analysis
|July 14, 2017
PubMed
Abstract

Insights

MicroRNA-211-5p (miR-211-5p) suppresses thyroid cancer (TC) growth by inhibiting SOX11. This study reveals miR-211-5p as a potential therapeutic target for TC, impacting cell viability, proliferation, and invasion.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Thyroid cancer (TC) remains a significant health concern.
  • Understanding the molecular mechanisms driving TC progression is crucial for developing effective treatments.

Purpose of the Study:

  • To investigate the relationship between microRNA-211-5p (miR-211-5p) and SOX11 in human thyroid cancer (TC) cells.
  • To determine the impact of their interaction on TC cell proliferation, viability, and invasion.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Dual luciferase reporter gene assay to confirm target interaction.
  • Flow cytometry, colony formation, Transwell, and MTT assays for cellular functions.
  • In vivo tumor formation assay in nude mice.

Main Results:

  • miR-211-5p was underexpressed, while SOX11 was overexpressed in TC tissues.
  • Overexpression of miR-211-5p inhibited SOX11 expression.
  • miR-211-5p suppressed TC cell cycle progression, proliferation, and invasion.
  • In vivo experiments confirmed the antitumor role of miR-211-5p.

Conclusions:

  • miR-211-5p negatively regulates SOX11 expression in TC.
  • This interaction significantly affects TC cell viability, proliferation, and invasion.
  • miR-211-5p demonstrates potential as a therapeutic agent for thyroid cancer.
Keywords:
SOX11in vivo experimentsmiR-211-5pthyroid cancer (TC)

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