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Updated: Feb 26, 2026

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS
Published on: March 14, 2013
Lipid profiling of complex biological mixtures by liquid chromatography/mass spectrometry using a novel scanning
Lee A Gethings1, Keith Richardson1, Jason Wildgoose1
1Waters Corporation, Stamford Avenue, Wilmslow, UK.
Rationale:
A novel data-independent acquisition method is detailed that incorporates a scanning quadrupole in front of an orthogonal acceleration time-of-flight (TOF) mass analyser. This approach is described and the attributes are compared and contrasted to other DIA approaches.
Methods:
Specific application of the method to both targeted and untargeted lipidomic identification strategies is discussed, with data from both shotgun and LC separated lipidomics experiments presented.
Results:
The benefits of the fast quadrupole scanning technique are highlighted, and include improvements in speed and specificity for complex mixtures providing high quality qualitative and quantitative data.
Conclusions:
In particular the high specificity afforded by the scanning quadrupole improves qualitative information for lipid identification.
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