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Related Experiment Video

Updated: Feb 10, 2026

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome

Published on: May 10, 2022

Pregnancy after human oocyte cryopreservation.

Lancet (London, England)

|April 19, 1986

Summary

Researchers report the first successful deep freezing and thawing of human oocytes, leading to a twin pregnancy. This breakthrough in oocyte cryopreservation offers new possibilities for fertility preservation.

Area of Science:

Reproductive biology
Cryobiology
Human embryology

Background:

Human oocyte cryopreservation is crucial for fertility preservation.
Previous attempts at oocyte freezing have faced challenges with survival and developmental potential.

Purpose of the Study:

To report the first successful deep freezing and thawing of human oocytes.
To achieve a viable pregnancy following oocyte cryopreservation and in vitro fertilization.

Main Methods:

Oocyte/cumulus-oophorus complex size reduction.
One-step addition of cryoprotectant dimethyl sulfoxide (DMSO).
Slow cooling with ice nucleation, rapid freezing to -196°C, and liquid nitrogen storage.

Main Results:

More Related Videos

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Published on: March 26, 2009

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Last Updated: Feb 10, 2026

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome

Laparoscopic Oocyte Retrieval and Cryopreservation during Vaginoplasty for Treatment of Mayer-Rokitansky-Kuster-Hauser Syndrome

Published on: May 10, 2022

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Using Mouse Oocytes to Assess Human Gene Function During Meiosis I

Published on: April 10, 2018

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Published on: March 26, 2009

80% of 40 oocytes showed morphological survival post-cryopreservation.
30 inseminated oocytes demonstrated 83% fertilization capacity.
60% of fertilized oocytes progressed to cleavage stage.

Conclusions:

Deep freezing and thawing of human oocytes is feasible.
Successful cryopreservation can lead to viable pregnancies.
This technique advances assisted reproductive technologies and fertility preservation options.