Physicochemical regulation of TGF and VEGF delivery from mesoporous calcium phosphate bone substitutes
Janina Möller-Siegert1, Julien Parmentier1, Patrice Laquerrière2
1Institut de Science des Matériaux de Mulhouse (IS2M), Université de Strasbourg, Université de Haute Alsace, UMR CNRS 7361, F-68057 Mulhouse, France.
Aim:
Determination of the physicochemical parameters governing growth factors (GFs) adsorption and release from mesoporous calcium phosphate ceramics.
Materials & Methods:
Six mesoporous calcium phosphate ceramics prepared by soft and hard templating were loaded with two different physiological concentrations of TGF-β1 or VEGF165 and their in vitro kinetics of adsorption/release were studied.
Results:
This low GF loading promotes adsorption on the highest binding sites. The usually encountered detrimental burst release is thus considerably reduced for samples prepared by hard-templating method.
Conclusion:
Our findings highlight that the strong affinity of GFs with the ceramic surfaces, demonstrated by a slow GFs release, is enhanced by the large surface area, confinement into mesopores of ceramics and high difference of surface charge between ceramic surfaces and GFs.
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