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Related Experiment Videos

Transglutaminase and neuronal differentiation.

R B Maccioni, N W Seeds

    Molecular and Cellular Biochemistry
    |February 1, 1986
    PubMed
    Summary
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    Cellular transglutaminase activity significantly increases during mouse brain maturation and neuroblastoma cell differentiation, particularly with neurite outgrowth. This enzyme may crosslink or modify tubulin, impacting microtubule assembly.

    Area of Science:

    • Neuroscience
    • Biochemistry
    • Cell Biology

    Background:

    • Cellular transglutaminase (TG) specific activity rises during mouse brain maturation.
    • TG activity increases significantly during neuroblastoma cell differentiation and neurite outgrowth.

    Purpose of the Study:

    • To investigate the role of TG in neuronal development and differentiation.
    • To identify TG substrates and its function in microtubule dynamics.

    Main Methods:

    • Measuring TG specific activity in developing mouse brains and differentiating neuroblastoma cells.
    • Identifying endogenous TG substrates using [3H]-putrescine.
    • Assessing TG activity on purified tubulin, microtubule-associated proteins, and microtubules.

    Main Results:

    Related Experiment Videos

    • TG specific activity increased 2.5-fold in mouse brain from day 3 to adulthood.
    • Neuroblastoma cells undergoing differentiation showed a 10-fold increase in TG activity.
    • Polymeric microtubules, but not tubulin alone, served as efficient acyl donor substrates for TG.
    • TG and putrescine stimulated microtubule assembly rate and extent.

    Conclusions:

    • TG activity is closely linked to neuronal differentiation and neurite formation.
    • TG may contribute to neuronal structure by crosslinking microtubules or modifying tubulin.
    • Post-translational modification of tubulin by TG could influence microtubule dynamics.