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Sexual dimorphisms of mRNA and miRNA in human/murine heart disease

Masato Tsuji1,2, Takanori Kawasaki3, Takeru Matsuda4

  • 1Division of Bio-informational Pharmacology, Medical Research Institute, Tokyo Medical Dental University, Tokyo, Japan.

Plos One
|July 14, 2017
PubMed

Insights

The male and female heart transcriptome shows significant sex differences in both healthy and diseased states. Understanding these genetic differences is key to developing gender-specific cardiac treatment strategies.

Area of Science:

  • Cardiovascular Biology
  • Genomics
  • Molecular Biology

Background:

  • Sexual dimorphisms are evident in cardiac diseases like ischemic cardiomyopathy (ICM), hypertrophic cardiomyopathy (HCM), and dilated cardiomyopathy (DCM).
  • Understanding sex-specific genetic programs is vital for tailored treatment strategies.
  • This study comprehensively analyzes sex differences in the heart transcriptome.

Purpose of the Study:

  • To characterize sexual dimorphisms in the healthy and diseased heart.
  • To investigate differences at the mRNA and miRNA transcriptome levels.
  • To lay the foundation for gender-specific cardiac treatment strategies.

Main Methods:

  • Meta-analysis and microarray analysis of existing mRNA data from mouse and human hearts.
  • Microarray analysis of miRNA from mouse and human heart samples (sham/MI and normal/ICM).
  • Integration of transcriptome data to identify sexually dimorphic genes and miRNAs.

Main Results:

  • Dozens/hundreds of sexually dimorphic genes identified in healthy and diseased hearts (ICM, HCM, DCM).
  • Sexually dimorphic genes are enriched in ontologies related to cardiac homeostasis.
  • Sexually dimorphic miRNAs identified in both mouse (MI and normal) and human (ICM and normal) hearts, revealing miRNA-mRNA networks with sex-biased operation.

Conclusions:

  • Significant sex differences exist in the mRNA and miRNA transcriptome of normal and diseased hearts.
  • These differences may impact cardiac homeostasis.
  • Provides a comprehensive genome-wide view of heart sexual dimorphisms, supporting gender-specific therapies.
Abstract

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