Related Experiment Video
Updated: Feb 26, 2026
Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol
Published on: March 25, 2020
Cyclodextrins increase phytosterol and tocopherol levels in suspension cultured cells obtained from mung beans and
Lorena Almagro1, Libertad Raquel Tudela1, Ana Belén Sabater-Jara1
1Dept. of Plant Biology, Faculty of Biology, University of Murcia, Campus de Espinardo, Murcia, E-30100, Spain.
Abstract:
In this work, suspension-cultured cells of mung beans and safflower were used in order to analyze the effect of methyl jasmonate and/or cyclodextrins, on bioactive compound production such as phytosterols and tocopherols. The results indicated that mung bean suspension-cultured cells produced higher amount of total phytosterols and tocopherols. In particular, mung bean suspension-cultured cells produced almost 220-fold higher levels of tocopherols than safflower suspension-cultured cells in the best conditions. However, while cyclodextrins were able to enhance extracellular production of phytosterols, in the case of tocopherols, they only increased their intracellular accumulation. Our results showed that mung bean cells could be used as a highly efficient system for the production of phytosterols and tocopherols which have a wide range of biological activities. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1662-1665, 2017.
