Host extracts induce changes in the proteome of plant pathogen Fusarium proliferatum

Fusarium proliferatum is a polyphagous pathogenic fungus able to infect many crop plants worldwide. Differences in proteins accumulated were observed when maize- and asparagus-derived F. proliferatum strains were exposed to host extracts prepared from asparagus, maize, garlic, and pineapple tissues. Seventy-three unique proteins were up-regulated in extract-supplemented cultures compared to the controls. They were all identified using mass spectrometry and their putative functions were assigned. A major part of identified proteins was involved in sugar metabolism and basic metabolic processes. Increased accumulation of proteins typically associated with stress response (heat shock proteins, superoxide dismutases, and glutaredoxins) as well as others, putatively involved in signal transduction, suggests that some metabolites present in plant extracts may act as elicitors inducing similar reaction as the abiotic stress factors. As a case study, thirteen genes encoding the proteins induced by the extracts were identified in the genomes of diverse F. proliferatum strains using gene-specific DNA markers. Extract-induced changes in the pathogen's metabolism are putatively a result of differential gene expression regulation. Our findings suggest that host plant metabolites present in the extracts can cause biotic stress resulting in elevated accumulation of diverse set of proteins, including those associated with pathogen's stress response.