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The 4-vessel Sampling Approach to Integrative Studies of Human Placental Physiology In Vivo
Published on: August 2, 2017
Proteomics analysis of human placenta reveals glutathione metabolism dysfunction as the underlying pathogenesis for
Xiaohan Jin1, Zhongwei Xu1, Jin Cao2
1Tianjin Key Laboratory of Cardiovascular Remodeling & Target Organ Injury, Pingjin Hospital Heart Center, Tianjin 300162, China; Central Laboratory, Logistics University of Chinese People's Armed Police Force, Tianjin 300309, China.
Insights
This study identified 145 altered proteins in placenta tissues from preeclampsia (PE) cases. Glutathione metabolism disorder in placental tissues may contribute to the development of PE.
Area of Science:
- Proteomics
- Biochemistry
- Reproductive Medicine
Background:
- Hypertensive disorder in pregnancy (HDP) encompasses conditions like preeclampsia (PE) and eclampsia.
- Preeclampsia poses significant risks during pregnancy.
Purpose of the Study:
- To identify differentially expressed proteins in placental tissues of preeclampsia (PE) cases.
- To investigate the role of protein expression changes in PE pathogenesis.
Main Methods:
- Utilized a 2D LC-MS/MS quantitative proteomics strategy.
- Quantified 2281 proteins and identified 145 differentially expressed proteins between PE and control cases (p<0.05).
Main Results:
- Bioinformatics analysis revealed proteins involved in oxidation-reduction, mitochondrion organization, and acute inflammatory response.
- Identified key molecules in glutamine metabolism and glutathione (GSH) regulation, including GPX1, GPX3, GSTs, SOD1, and GCLM.
- Highlighted the involvement of glutathione, mercapturic acid, and arginine metabolism in the GSSG to GSH conversion process.
Conclusions:
- Placental glutathione metabolism disorder is implicated in the pathogenesis of preeclampsia (PE).
- These findings offer potential targets for understanding and managing PE.
Abstract:
Hypertensive disorder in pregnancy (HDP) refers to a series of diseases that cause the hypertension during pregnancy, including HDP, preeclampsia (PE) and eclampsia. This study screens differentially expressed proteins of placenta tissues in PE cases using 2D LC-MS/MS quantitative proteomics strategy. A total of 2281 proteins are quantified, of these, 145 altering expression proteins are successfully screened between PE and control cases (p<0.05). Bioinformatics analysis suggests that these proteins are mainly involved in many biological processes, such as oxidation reduction, mitochondrion organization, and acute inflammatory response. Especially, the glutamine metabolic process related molecules, GPX1, GPX3, SMS, GGCT, GSTK1, NFκB, GSTT2, SOD1 and GCLM, are involved in the switching process from oxidized glutathione (GSSG) conversion to the reduced glutathione (GSH) by glutathione, mercapturic acid and arginine metabolism process. Results of this study revealed that glutathione metabolism disorder of placenta tissues may contribute to the occurrence of PE disease.
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