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Complete Genome Sequence of Streptococcus pneumoniae Virulent Phage MS1
Witold Kot1, Mourad Sabri2, Hélène Gingras3
1Department of Environmental Science, Aarhus University, Roskilde, Denmark.
Genome Announcements
|July 15, 2017
Abstract:
The lytic Streptococcus pneumoniae phage MS1 was isolated from a throat swab of a patient with symptoms of upper respiratory tract infection. The genome of this siphophage has 56,075 bp, 42.3% G+C content, and 77 open reading frames, including queuosine biosynthesis genes. Phage MS1 is related to pneumococcal phage Dp-1.
Insights
A novel lytic bacteriophage, MS1, targeting Streptococcus pneumoniae was identified. Its genome analysis revealed 77 open reading frames, including essential queuosine biosynthesis genes, indicating potential therapeutic applications.
Area of Science:
- Microbiology and Virology
- Bacteriophage Research
- Genomics
Background:
- Streptococcus pneumoniae is a significant human pathogen causing various infections.
- Bacteriophages (phages) are viruses that infect bacteria and show promise as therapeutic agents.
- Isolation and characterization of novel phages are crucial for developing phage therapy.
Purpose of the Study:
- To isolate and characterize a lytic bacteriophage targeting Streptococcus pneumoniae.
- To analyze the genomic features of the isolated phage MS1.
- To investigate the phage's relationship with other known pneumococcal phages.
Main Methods:
- Isolation of bacteriophage MS1 from a patient's throat swab sample.
- Genome sequencing and analysis of bacteriophage MS1.
- Bioinformatic analysis to identify open reading frames and related phages.
Main Results:
- The lytic phage MS1 was successfully isolated from a patient with upper respiratory tract infection.
- The complete genome of phage MS1 comprises 56,075 base pairs with 42.3% G+C content.
- The genome contains 77 open reading frames, including genes involved in queuosine biosynthesis, and is related to pneumococcal phage Dp-1.
Conclusions:
- Phage MS1 is a novel lytic bacteriophage with potential for targeting Streptococcus pneumoniae.
- The genomic information, including queuosine biosynthesis genes, provides insights into phage biology.
- The relationship to Dp-1 suggests potential shared characteristics and evolutionary links within pneumococcal phages.
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