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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Feb 26, 2026

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Patch-Clamp Techniques for Single Endolysosomal Vesicle Analysis

Published on: April 4, 2025

Patch clamp-assisted single neuron lipidomics.

Collin B Merrill¹, Abdul Basit², Andrea Armirotti²

¹Department of Anatomy and Neurobiology, University of California, Irvine, Irvine, CA, 92697, USA.

Scientific Reports

|July 15, 2017

Summary

This summary is machine-generated.

Researchers developed a new method to analyze lipids in single brain cells. This technique reveals differences in lipid profiles between neuron types and states, advancing our understanding of brain lipidomics.

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Area of Science:

Neuroscience
Lipidomics
Cell Biology

Background:

Understanding brain lipid functions requires cellular resolution analysis.
Current methods lack the ability to analyze lipids at the single-neuron level.

Purpose of the Study:

To present a novel method for detecting lipids within identified single neurons from live mammalian brains.
To explore lipid profiles in specific hippocampal neuron types and under different physiological conditions.

Main Methods:

Single neuronal cell bodies were captured using patch clamping from perfused mouse brain slices.
Lipids were analyzed via an optimized nanoflow liquid chromatography/mass spectrometry (LC/MS) protocol.
The method enables lipid detection in identified single neurons from live mammalian brains.

Main Results:

Over 40 lipid species were identified in dentate gyrus granule cells and CA1 pyramidal neurons.
Significant differences in lipid profiles were observed between single neurons and whole brain tissue.
Distinct lipid profiles were found between resting and physiologically stimulated neurons.

Conclusions:

Patch clamp-assisted single neuron lipidomics is a viable method for detailed lipid analysis.
This approach can reveal crucial insights into neuronal lipid homeostasis in health and disease.
The technique offers broad applicability for neuroscience research.