Matrix coating assisted by an electric field (MCAEF) for enhanced tissue imaging by MALDI-MS
Xiaodong Wang1, Jun Han1, Juncong Yang1
1University of Victoria - Genome British Columbia Proteomics Centre , Vancouver Island Technology Park, #3101-4464 Markham St. , Victoria , BC V8Z 7X8 , Canada . Email: christoph@proteincentre.com ; ; Tel: +1-250-483-3221.
Chemical Science
|July 15, 2017
Summary
A new method called matrix coating assisted by an electric field (MCAEF) improves tissue imaging sensitivity and analyte detection in mass spectrometry. This technique enhances lipid and protein detection for better MALDI imaging results.
Area of Science:
- Analytical Chemistry
- Biotechnology
- Mass Spectrometry Imaging
Background:
- Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a powerful tool for tissue imaging.
- Enhancing sensitivity and analyte coverage in MALDI-MS imaging remains a key challenge.
Purpose of the Study:
- To develop and evaluate a novel technique, matrix coating assisted by an electric field (MCAEF), for improving MALDI-MS tissue imaging.
- To demonstrate the enhanced sensitivity and analyte detection capabilities of MCAEF.
Main Methods:
- Applied a static and uniform electric field during matrix spray-coating of tissue sections.
- Analyzed various tissues (rat liver, brain, porcine adrenal gland) using different MALDI matrices.
- Utilized MALDI-Fourier transform ion cyclotron resonance MS and MALDI-time-of-flight MS for lipid and protein imaging.
Main Results:
- MCAEF significantly increased the sensitivity of lipid and protein detection.
- Observed successful imaging of a larger number of analytes across diverse tissues and matrices.
- Demonstrated a substantial increase in identified lipids (648 vs. 344) and detected protein signals (232 vs. 119) in rat brain tissue.
- Enabled the detection of higher molecular-weight proteins.
Conclusions:
- MCAEF offers significant performance improvements for MALDI tissue imaging.
- The technique is independent of solvent composition and pH, and applicable to various tissues and matrices.
- MCAEF shows potential to become a standard method for enhanced MALDI imaging.
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