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An Efficient and Simple Method to Establish NK and T Cell Lines from Patients with Chronic Active Epstein-Barr Virus Infection
Published on: March 30, 2018
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Epstein-Barr virus lytic cycle involvement in diffuse large B cell lymphoma.
Melina Cohen1,2, Aldana Georgina Vistarop1,2, Fuad Huaman3
1Molecular Biology Laboratory, Pathology Division, Ricardo Gutiérrez Children's Hospital, Buenos Aires, Argentina.
Hematological Oncology
|July 15, 2017
Summary
Epstein-Barr virus (EBV) lytic replication plays a role in diffuse large B cell lymphoma (DLBCL) pathogenesis. Lytic gene expression correlated with latent genes and immune markers, suggesting therapeutic potential.
Area of Science:
- Virology
- Oncology
- Immunology
Background:
- Epstein-Barr virus (EBV) is linked to B cell transformation, primarily via latent proteins.
- Recent evidence suggests a role for EBV lytic replication in early lymphomagenesis.
- The EBV lytic cycle in diffuse large B cell lymphoma (DLBCL) remains largely unexplored.
Purpose of the Study:
- To investigate the contribution of EBV lytic replication to lymphomagenesis.
- To evaluate early and late EBV lytic gene expression in EBV+ DLBCL.
- To correlate lytic gene expression with immune response markers.
Main Methods:
- Real-time quantitative polymerase chain reaction (qPCR) for viral lytic transcripts.
- Immunohistochemical staining for early lytic protein BMRF1.
- Correlation analysis between viral gene expression and immune cell counts/cytokines.
Main Results:
- An unexpected number of EBV+ DLBCL cells expressed lytic transcripts (BZLF1, BHRF1, BLLF1).
- Lytic antigen expression was confirmed by BMRF1 staining.
- Positive correlation found between lytic and latent EBV gene expression.
- BZLF1 expression negatively correlated with CD4 cell counts.
- BLLF1 expression positively correlated with IFNγ.
- Interleukin 10 (IL-10) transcripts positively correlated with lytic gene expression.
Conclusions:
- EBV lytic and latent gene expressions are closely linked in DLBCL pathogenesis.
- Immune responses, including CD4 counts, IFNγ, and IL-10, are associated with EBV lytic activity.
- Complete lytic reactivation could potentially lead to EBV-positive tumor cell killing, offering a therapeutic strategy.
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