Related Experiment Video

Updated: Feb 26, 2026

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues
12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.4K

MiR-411 functions as a tumor suppressor in renal cell cancer

Xintao Zhang1,2,3, Meng Zhang1,2,4, Jianli Cheng3

  • 1Department of Urology, Shenzhen Second People's Hospital, Clinical Medicine College of Anhui Medical University, Shenzhen, Guangdong - PR China.

The International Journal of Biological Markers
|July 15, 2017
PubMed
Abstract

Insights

MicroRNA-411 (miR-411) acts as a tumor suppressor in renal cell carcinoma (RCC). This study found miR-411 is down-regulated in RCC, suppressing cancer cell proliferation and migration while inducing apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) are key regulators in tumorigenesis, acting as oncogenes or tumor suppressors.
  • Previous studies indicated miR-411 is downregulated in renal cell carcinoma (RCC).
  • The specific role of miR-411 in RCC pathogenesis requires further investigation.

Purpose of the Study:

  • To investigate the expression levels of miR-411 in RCC tissues and cell lines.
  • To determine the functional role of miR-411 in regulating RCC cell proliferation, apoptosis, and migration.
  • To assess the impact of miR-411 restoration on normal renal cells.

Main Methods:

  • Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) to measure miR-411 expression.
  • Cell proliferation assays (MTT, CCK-8) and migration assays (scratch wound, transwell) following miR-411 mimic transfection.
  • Flow cytometry to analyze apoptosis induction by miR-411.

Main Results:

  • miR-411 expression was significantly lower in RCC tissues and cell lines compared to normal controls.
  • Overexpression of miR-411 inhibited proliferation and migration in RCC cell lines (786-O, ACHN).
  • miR-411 induced apoptosis in RCC cells but had no significant effect on normal renal cells (293T).

Conclusions:

  • miR-411 functions as a tumor suppressor in renal cell carcinoma.
  • The downregulation of miR-411 contributes to RCC development.
  • Further research is needed to elucidate miR-411's mechanisms and identify its target genes in RCC.

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K
MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR08:30

MicroRNA Detection in Prostate Tumors by Quantitative Real-time PCR qPCR

Published on: May 16, 2012

25.1K

Related Concept Videos

Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.8K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K