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Updated: Aug 15, 2026

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles
Published on: November 1, 2012
Enhanced contractile activity of goldfish skeletal muscle related to cold acclimation
Abstract:
Dorsal muscles were isolated from goldfish acclimated to 8 degrees C and 25 degrees C, and stimulated electrically at 5-35 degrees C. Contractile activity of isolated muscle from the 8 degrees C fish was highest at 15 degrees C, whilst that of the 25 degrees C fish was independent of experimental temperature. The activity was significantly increased with cold acclimation. Pyruvate and lactate contents of red and white muscles increased with work. Addition of iodoacetic acid into the incubation medium caused a decrease of lactate production and contractile activity in muscle from the 8 degrees C fish, suggesting that the increase of the activity was partly associated with an increased activity of anaerobic glycolysis of the tissues.
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