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Related Concept Videos

Confocal Fluorescence Microscopy01:16

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Multimodal nonlinear microscope based on a compact fiber-format laser source.

Francesco Crisafi1, Vikas Kumar1, Antonio Perri1

  • 1IFN-CNR, Dipartimento di Fisica, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milano, Italy.

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
|July 15, 2017
PubMed
Summary
This summary is machine-generated.

A new multimodal non-linear optical (NLO) microscope integrates coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS), and two-photon-excitation fluorescence (TPEF) for advanced imaging. This flexible NLO microscopy platform enables simultaneous visualization of biological samples and microparticles.

Keywords:
CARSCoherent Raman spectroscopyMultimodalNonlinear optical microscopySRSTPEF

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Area of Science:

  • Optics and Photonics
  • Microscopy
  • Biomedical Imaging

Background:

  • Non-linear optical (NLO) microscopy offers advanced imaging capabilities.
  • Integrating multiple NLO modalities onto a single platform presents technical challenges.
  • Existing NLO microscopes may lack flexibility and accessibility for diverse applications.

Purpose of the Study:

  • To develop and present a versatile, multimodal NLO laser-scanning microscope.
  • To integrate coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS), and two-photon-excitation fluorescence (TPEF) into a single system.
  • To demonstrate the microscope's utility for imaging microparticles and biological specimens.

Main Methods:

  • A compact fiber-format laser was employed as the excitation source.
  • Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS), and two-photon-excitation fluorescence (TPEF) were integrated.
  • The NLO microscope was constructed using off-the-shelf components for accessibility and re-configurability.

Main Results:

  • Simultaneous CARS and SRS imaging of poly(methyl methacrylate) and polystyrene beads was achieved.
  • SRS imaging visualized the cell walls of an Elodea aquatic plant.
  • TPEF imaging revealed chloroplasts within the Elodea plant sample.
  • The developed NLO microscope demonstrated high flexibility and ease of reconfiguration.

Conclusions:

  • The presented multimodal NLO microscope provides a flexible and accessible platform for advanced imaging.
  • The integrated CARS, SRS, and TPEF modalities enable comprehensive visualization of microparticles and biological structures.
  • This NLO microscopy system facilitates diverse research applications in optics and life sciences.