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CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

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CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

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Updated: Feb 26, 2026

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

Identifying synthetic lethal targets using CRISPR/Cas9 system.

Jaspreet Kaur Dhanjal¹, Navaneethan Radhakrishnan¹, Durai Sundar¹

¹Department of Biochemical Engineering and Biotechnology, DBT-AIST International Laboratory for Advanced Biomedicine (DAILAB), Indian Institute of Technology Delhi, Hauz Khas, New Delhi 110016, India.

Methods (San Diego, Calif.)

|July 16, 2017

Summary

This summary is machine-generated.

CRISPR/Cas9 technology enables efficient synthetic lethality screening. This review details designing CRISPR screens, selecting cell types, and optimal pooled negative selection strategies for identifying synthetic lethal targets.

Keywords:

Arrayed screen CRISPR/Cas9 Knockout library Pooled screen Synthetic lethality

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CIRCLE-Seq for Interrogation of Off-Target Gene Editing

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

Area of Science:

Genomics
Molecular Biology
Cancer Research

Background:

Synthetic lethality is a genetic interaction where two events are lethal together.
High-throughput screening traditionally used chemical compounds but now favors RNA interference (RNAi).
CRISPR/Cas9 gene editing offers a precise and efficient method for genetic screening.

Purpose of the Study:

To outline key considerations for designing CRISPR/Cas9-based screening experiments.
To identify synthetic lethal targets through genome-wide screening.
To compare CRISPR/Cas9 with RNAi technologies for synthetic lethality studies.

Main Methods:

Utilizing CRISPR/Cas9 knockout libraries for complete gene knockdown.
Employing haploid cells with deficient DNA repair for enhanced loss-of-function screens.
Comparing arrayed versus pooled screening formats, with a focus on pooled negative selection.

Main Results:

CRISPR/Cas9 facilitates efficient genome-wide knockout screening.
Pooled screening with negative selection is identified as the optimal strategy.
CRISPR/Cas9 offers advantages over traditional RNAi methods for target identification.

Conclusions:

CRISPR/Cas9 is a powerful tool for discovering synthetic lethal interactions.
Careful experimental design, including library choice and cell type selection, is crucial.
Computational approaches aid in identifying synthetic lethal interactions from screening data.