Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxygen Transport in the Blood01:27

Oxygen Transport in the Blood

7.4K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
7.4K
Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors01:28

Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors

659
Phosphodiesterase 5 (PDE5) inhibitors are potent enzymes that function to hydrolyze cyclic nucleotides to their corresponding 5' monophosphates. Their unique biochemical properties have been applied in treating Pulmonary Arterial Hypertension (PAH).
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
659
Adrenergic Agonists: Chemistry and Structure-Activity Relationship01:16

Adrenergic Agonists: Chemistry and Structure-Activity Relationship

4.0K
Adrenergic agonists' structure-activity relationship (SAR) determines their selectivity and efficacy. These agonists comprise a phenylethylamine moiety with an aromatic ring and an ethylamine side chain.
Aromatic ring substitutions: Substituting the aromatic ring with –OH groups at positions 3 and 4 yields catecholamines (e.g., epinephrine), which have a high affinity for adrenoceptors. Hydrogen bonding between –OH groups and receptors enhances adrenergic activity.
Separation of...
4.0K
Adrenergic Antagonists: ɑ and β-Receptor Blockers01:31

Adrenergic Antagonists: ɑ and β-Receptor Blockers

1.3K
Third-generation β-blockers, such as labetalol and carvedilol, represent a significant advancement in managing cardiovascular conditions. Unlike conventional β-blockers, which can induce peripheral vasoconstriction, third-generation drugs block α1 adrenoceptors. This promotes vasodilation through several mechanisms, such as increased nitric oxide production, inhibition of calcium ion entry, opening of potassium ion channels, and antioxidant action. Labetalol, for instance, is...
1.3K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

4.5K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
4.5K
Treatment for Pulmonary Arterial Hypertension: Prostacyclin Receptor Agonists01:23

Treatment for Pulmonary Arterial Hypertension: Prostacyclin Receptor Agonists

546
Prostacyclin receptor agonists are a class of therapeutic agents integral to managing pulmonary arterial hypertension (PAH). These drugs operate by mimicking the action of prostaglandin I2, or PGI2, a naturally occurring compound in the body.
These agonists bind to the IPR receptor situated on the plasma membrane of the pulmonary artery smooth muscle cells. This binding triggers a cascade of reactions known as the GS-AC-cAMP-PKA pathway. This pathway results in the relaxation of smooth muscle...
546

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Interference effects in H<sub>2</sub>O<sub>2</sub> quantification: A comparative study of three common methods in photocatalytic systems.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

Desmearing Bonse-Hart USANS data using Bayesian Gaussian process regression.

The Journal of chemical physics·2026
Same author

Programmable Electrostatics in Charge-Patterned Polypeptoid Micelles Probed by Small-Angle Neutron Scattering.

Macromolecules·2026
Same author

An algebraic convolution formulation for multiple-scattering correction in small-angle neutron scattering.

The Journal of chemical physics·2026
Same author

A Bayesian desmearing algorithm for Bonse-Hart USANS with anisotropic scattering.

The Journal of chemical physics·2026
Same author

Incense Aerosol-Induced Neurotoxicity Disrupts α-Synuclein Homeostasis in a Cellular Parkinson's Disease Model, Distinct from Cigarette Aerosols.

Chemical research in toxicology·2026
Same journal

Serum vitamin D level and its association with vertigo frequency and severity in Meniere disease.

Scientific reports·2026
Same journal

PFA-Net: a physics-informed feature enhancement and attention network for interpretable bearing fault diagnosis under strong noise.

Scientific reports·2026
Same journal

Circulating inflammatory, redox, and apoptosis-related alterations in drug-naive idiopathic pulmonary fibrosis: an exploratory case-control study.

Scientific reports·2026
Same journal

A baseline-oriented dynamic aggregation approach for demand-side heterogeneous controllable resources.

Scientific reports·2026
Same journal

Temporal precision and accuracy in schizophrenia: an exploratory study.

Scientific reports·2026
Same journal

Prefrontal EEG spectral and nonlinear signatures of subthreshold depression during resting state and affectively valenced picture/video viewing: a participant-level analysis.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Feb 26, 2026

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
08:23

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

10.4K

Phthalide Derivatives from Angelica Sinensis Decrease Hemoglobin Oxygen Affinity: A New Allosteric-Modulating

Wei-Ren Chen1, Youqing Yu1, Muhammad Zulfajri1

  • 1Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan, 80424, Republic of China.

Scientific Reports
|July 16, 2017
PubMed
Summary
This summary is machine-generated.

Angelica sinensis (AS) and its phthalides reduce hemoglobin's oxygen affinity by stabilizing its T-state. This mechanism, involving 2,3-BPG synergy, may explain AS's diverse therapeutic effects.

More Related Videos

Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats
09:47

Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats

Published on: June 14, 2024

1.2K
Preparation of N-2-alkoxyvinylsulfonamides from N-tosyl-1,2,3-triazoles and Subsequent Conversion to Substituted Phthalans and Phenethylamines
10:42

Preparation of N-2-alkoxyvinylsulfonamides from N-tosyl-1,2,3-triazoles and Subsequent Conversion to Substituted Phthalans and Phenethylamines

Published on: January 3, 2018

10.3K

Related Experiment Videos

Last Updated: Feb 26, 2026

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry
08:23

Characterization of Sickling During Controlled Automated Deoxygenation with Oxygen Gradient Ektacytometry

Published on: November 5, 2019

10.4K
Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats
09:47

Author Spotlight: Utilizing Next-Generation Polymerized Human Hemoglobin for Improved Donor Lung Evaluation and Preservation in Rats

Published on: June 14, 2024

1.2K
Preparation of N-2-alkoxyvinylsulfonamides from N-tosyl-1,2,3-triazoles and Subsequent Conversion to Substituted Phthalans and Phenethylamines
10:42

Preparation of N-2-alkoxyvinylsulfonamides from N-tosyl-1,2,3-triazoles and Subsequent Conversion to Substituted Phthalans and Phenethylamines

Published on: January 3, 2018

10.3K

Area of Science:

  • Pharmacology
  • Biochemistry
  • Herbal Medicine

Background:

  • Angelica sinensis (AS) is a traditional herbal medicine with diverse reported therapeutic effects, including anti-hypertensive and anti-cancer properties.
  • The molecular mechanisms underlying AS's broad efficacies remain largely unknown.
  • Hemoglobin (Hb) plays a crucial role in oxygen transport, and its function can be modulated.

Purpose of the Study:

  • To investigate the effects of AS and its phthalide derivatives on hemoglobin structure and function.
  • To elucidate the molecular interactions between phthalides and hemoglobin.
  • To propose a mechanism for how AS influences oxygen transport.

Main Methods:

  • Spectroscopy
  • Oxygen equilibrium experiments
  • Docking modeling

Main Results:

  • AS and its phthalides decrease hemoglobin's oxygen affinity by inhibiting the transition to the high-affinity R-state.
  • Phthalides act synergistically with 2,3-bisphosphoglycerate (2,3-BPG), a natural Hb modulator.
  • Docking studies suggest phthalides bind at the α1/α2 interface, stabilizing the T-state through salt bridges.

Conclusions:

  • A novel allosteric-modulating mechanism for phthalides is proposed, facilitating Hb oxygen transport.
  • This mechanism may correlate with the therapeutic activities of Angelica sinensis.
  • Phthalides show potential as 2,3-BPG substitutes or supplements, with implications for systemic biology and preventive medicine.