Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere...

To explore the epigenetic mechanisms regulating mesenchymal stem cells, we analyzed epigenetic patterns in control goat fetal fibroblast cells (gFFCs), adipose-derived stem cells (gADSCs), bone marrow stromal cells (gBMSCs), and muscle-derived satellite cells (gMDSCs). We found that the 5mC content of gBMSC genomes was lower than that of gFFC genomes, while the 5mC content of gADSC and gMDSC genomes surpassed that of gFFC genomes. H3K9 acetylation did not differ significantly among those cells; gFFCs, gADSCs, and gMDSCs contained acetylated H3K9, H3K14, H3K18, H4K5, and H4K12, but gBMSCs contained almost no acetylated H4K5 and H4K12. DNMT1, DNMT3A, and DNMT3B expression levels in gBMSCs and gMDSCs were relatively high; TET1 and TET2 expression levels in gFFCs, gADSCs, gBMSCs, and gMDSCs were relatively low; the TET3 expression level was relatively high, but was not statistically significant. The expression levels of HDAC1, HDAC6, SIRT1, Tip60, and PCAF in gADSCs, gBMSCs, and gMDSCs were higher than those in gFFCs; this observation was consistent with the real-time quantitative PCR results. P300 expression was not detected. We found that epigenetic modification was active in mesenchymal stem cells, which benefited the regulation of these cells.