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Virulent and pathogenic features on the Cronobacter sakazakii polymyxin resistant pmr mutant strain s-3
Xuerui Bao1, Ling Yang2, Lequn Chen1
1School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Abstract:
Cronobacter sakazakii is a well-known opportunistic pathogen responsible for necrotizing enterocolitis, meningitis and septicaemia in the premature, immunocompromised infants and neonates. This pathogen possesses various virulence factors and regulatory systems, and pmrA/pmrB regulatory system has been identified in a variety of bacterial species. The current study aims to investigate role of pmrA gene in the pathogenicity and virulence characteristics of Cronobacter sakazakii using whole genome sequencing and RNA-seq. Results demonstrated that the absence of pmrA has the potential to affect Cronobacter sakazakii on its pathogenicity, virulence and resistance abilities by regulating expression of numerous related genes, including CusB, CusC, CusR and ESA_pESA3p05434.
Insights
The pmrA gene significantly impacts Cronobacter sakazakii virulence and pathogenicity. Its absence affects bacterial resistance and the expression of key virulence-related genes in this opportunistic pathogen.
Area of Science:
- Microbiology
- Bacterial Pathogenesis
- Genomics
Background:
- Cronobacter sakazakii is an opportunistic pathogen causing severe infections in vulnerable infants.
- The pmrA/pmrB regulatory system is implicated in virulence across various bacterial species.
Purpose of the Study:
- To investigate the role of the pmrA gene in Cronobacter sakazakii pathogenicity and virulence.
- To understand how pmrA influences bacterial resistance and gene expression.
Main Methods:
- Whole genome sequencing was employed to analyze Cronobacter sakazakii.
- RNA-sequencing (RNA-seq) was used to assess gene expression changes.
Main Results:
- The absence of the pmrA gene was shown to affect Cronobacter sakazakii's pathogenicity.
- Virulence and resistance capabilities were altered in pmrA-deficient strains.
- Expression of genes such as CusB, CusC, CusR, and ESA_pESA3p05434 was regulated by pmrA.
Conclusions:
- The pmrA gene plays a crucial role in Cronobacter sakazakii virulence and pathogenicity.
- Targeting pmrA could be a strategy to control Cronobacter sakazakii infections.
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