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Updated: Feb 26, 2026

Author Spotlight: Point-of-Care Ultrasound for Gastric Content Assessment and Risk Stratification in Perioperative Care
Published on: September 22, 2023
Normal ultrasonographic reference values for the gastrointestinal tract in developing puppies
Tommaso Banzato1, Chiara Milani1, Elena Zambello1
1Department of Animal Medicine, Production and Health, University of Padua, Viale dell'Università 16, AGRIPOLIS, Legnaro, 35020 Padua, Italy.
Ultrasonography revealed that gastrointestinal tract wall thickness increases with age in developing puppies. Body weight significantly impacts duodenal and jejunal thickness more than stomach and colon thickness.
Area of Science:
- Veterinary Medicine
- Comparative Anatomy
- Diagnostic Imaging
Background:
- Assessing gastrointestinal wall thickness is crucial for understanding canine development.
- Ultrasonography offers a non-invasive method for evaluating organ structure.
Purpose of the Study:
- To establish reference intervals for ultrasonographic gastrointestinal wall thickness in developing puppies.
- To investigate the influence of age and body weight on these measurements.
Main Methods:
- Ultrasonography was used to measure the thickness of stomach, duodenum, jejunum, and colon wall layers.
- Measurements were taken in puppies at 4, 8, and 16 weeks of age.
Main Results:
- Gastrointestinal wall thickness increased with age across all examined organs.
- Body weight had a stronger correlation with duodenal and jejunal thickness compared to the stomach and colon.
- A strong correlation was observed between body weight and the mucosal layer thickness of the duodenum and jejunum.
Conclusions:
- Age- and body weight-specific reference intervals for canine gastrointestinal wall thickness were established.
- These findings provide valuable data for diagnosing developmental abnormalities in puppies.
- Gender did not significantly affect gastrointestinal wall thickness in the studied population.
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