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Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Homologous Recombination02:31

Homologous Recombination

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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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Updated: Feb 26, 2026

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

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CRISPR/Cas9-Based Genome Editing in Plants.

Yaling Zhang1, Xingliang Ma2, Xianrong Xie1

  • 1State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou, China; College of Life Sciences, South China Agricultural University, Guangzhou, China.

Progress in Molecular Biology and Translational Science
|July 18, 2017
PubMed
Summary
This summary is machine-generated.

The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system offers a simple and efficient genome editing tool for plants. This technology is revolutionizing plant science research, functional genomics, and genetic improvement.

Keywords:
CRISPR/Cas9DSDecodeGenome editingPlant

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Area of Science:

  • Plant Science
  • Genetics
  • Molecular Biology

Background:

  • Genome editing technologies are advancing rapidly in plant science.
  • The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system is a next-generation genome editing tool.
  • CRISPR/Cas9 is increasingly replacing older technologies like zinc finger nucleases and transcription activator-like effector nucleases.

Purpose of the Study:

  • To introduce the technical features of the plant CRISPR/Cas9 genome editing system.
  • To highlight the applications of CRISPR/Cas9 in plant functional genomics.
  • To discuss the use of CRISPR/Cas9 for plant genetic improvement.

Main Methods:

  • Review of CRISPR/Cas9 technology features.
  • Analysis of CRISPR/Cas9 applications in plant research.
  • Discussion of CRISPR/Cas9's role in genetic improvement.

Main Results:

  • CRISPR/Cas9 offers significant advantages in simplicity and efficiency for plant genome editing.
  • The system is a powerful tool for plant functional genomics studies.
  • CRISPR/Cas9 facilitates effective genetic improvement in plants.

Conclusions:

  • The CRISPR/Cas9 system is a highly efficient and simple genome editing tool for plants.
  • It is a transformative technology for plant functional genomics and genetic improvement.