Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Arsenic detoxification within thermo-alkaline biofilms.

Frontiers in microbiology·2026

Same author

Impact of the gut microbiome on health impacts of Haskap berries: study protocol for a randomized control trial.

Trials·2026

Same author

The Impact of Forever Chemicals on Protein Structure and Function.

International journal of molecular sciences·2026

Same author

Differential Metabolic Changes in Zebrafish Embryos Are Induced by Discontinued Citalopram Exposure.

Biomedicines·2026

Same author

Tissue-Specific and Spatially Dependent Metabolic Signatures Perturbed by Injury in Male and Female Mice.

Arthritis & rheumatology (Hoboken, N.J.)·2026

Same author

Beyond Repression: ArsR Functions as a Global Activator of Metabolic and Redox Responses in <i>Escherichia coli</i>.

Proteomes·2026

Search research articles

Related Experiment Video

Updated: Feb 26, 2026

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Analysis of Raw Biofluids by Mass Spectrometry Using Microfluidic Diffusion-Based Separation.

Joshua Heinemann^1,2,3, Brigit Noon¹, Daniel Willems¹

¹Department of Chemistry and Biochemistry, Montana State University, Bozeman, MT 59717.

Analytical Methods : Advancing Methods and Applications

|July 18, 2017

Summary

This summary is machine-generated.

A novel metabolite extraction chip (MEC) enables high-throughput analysis of biomarkers in biofluids. This cost-effective system advances personalized healthcare through frequent, routine testing for disease monitoring.

More Related Videos

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

Related Experiment Videos

Last Updated: Feb 26, 2026

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice

Published on: November 23, 2015

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Author Spotlight: Characterization of Low-Affinity Protein Interactions in Solution Using MassFluidix Technology

Published on: January 26, 2024

Area of Science:

Biomedical Engineering
Analytical Chemistry
Metabolomics

Background:

Biomarker monitoring is crucial for understanding disease, aging, and addiction, but high costs limit frequent testing.
Longitudinal analyses offer biological insights but are hindered by the expense of replicate testing.
Personalized healthcare and early disease detection require more frequent and accessible biomarker analysis.

Purpose of the Study:

To develop a cost-effective system for measuring metabolite abundance in raw biofluids.
To adapt existing microfluidic technology for high-throughput screening applications.
To demonstrate the utility of the metabolite extraction chip (MEC) for analyzing human biofluids.

Main Methods:

Utilized a metabolite extraction chip (MEC) based on microfluidic diffusive extraction of small molecules and metabolites.
Adapted the MEC for high-throughput screening with standard liquid chromatography-mass spectrometry (LC-MS) instrumentation.
Performed quantitative analysis of clinical predictors such as nicotine, caffeine, and glutathione in human biofluids.

Main Results:

Demonstrated that the MEC can measure biologically relevant markers in blood and urine.
Showcased the adaptation of the MEC for high-throughput screening, enabling cost-effective metabolite analysis.
Provided insights into the sensitivity and application of the system for analyzing human biofluids.

Conclusions:

The developed MEC system offers a sensitive and efficient method for metabolite quantification in biofluids.
This technology facilitates high-throughput screening, potentially reducing healthcare costs and enabling personalized medicine.
The MEC system supports frequent routine testing, deepening the understanding of disease emergence and progression.