A Label-Free Fluorescent Array Sensor Utilizing Liposome Encapsulating Calcein for Discriminating Target Proteins by...

Area of Science:

• Biotechnology
• Analytical Chemistry
• Materials Science

Background:

• Traditional fluorescent array sensors often rely on labeling, which can complicate detection processes.
• Developing label-free biosensing platforms is crucial for simplifying protein analysis and reducing experimental steps.

Purpose of the Study:

• To develop a novel label-free fluorescent arrayed biosensor for discriminating protein species and concentrations.
• To utilize differences in fluorescent molecule permeation through liposome membranes to modulate protein interactions.

Main Methods:

• Employing diverse phospholipid liposome species encapsulating fluorescent molecules.
• Modulating liposome-target protein interactions by controlling fluorescent molecule permeation.
• Analyzing fluorescence emission intensities using a CMOS imager and Principal Component Analysis (PCA).

Main Results:

• Confirmed high fluorescence emission intensity correlated with fluorescent molecule concentration upon leakage from perturbed liposomes.
• Successfully discriminated different protein species and concentrations using varied lipid membranes via PCA.
• Demonstrated higher discrimination accuracy with a single-shot array sensor compared to a multi-shot single sensor.

Conclusions:

• The developed fluorescent arrayed biosensor provides a novel label-free approach for protein detection.
• The sensor effectively distinguishes protein species and concentrations based on liposome-protein interactions.
• This method offers enhanced accuracy and efficiency for biosensing applications.