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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Feb 26, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy (ATOM).

Anson H L Tang¹, Queenie T K Lai¹, Bob M F Chung²

¹Department of Electrical and Electronic Engineering, The University of Hong Kong.

Journal of Visualized Experiments : Jove

|July 18, 2017

Summary

This summary is machine-generated.

Asymmetric-detection time-stretch optical microscopy (ATOM) enables high-throughput imaging flow cytometry. This advanced technique provides high-contrast, sub-cellular resolution images of unlabeled cells, overcoming throughput limitations.

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Area of Science:

Biophotonics
Cellular Imaging
Flow Cytometry

Background:

Flow cytometry advancements focus on increasing measurable parameters for multidimensional data analysis and higher-confidence results.
High-resolution imaging in flow cytometry enables complex morphological analysis of cellular structures, crucial for understanding cellular functions.
Current imaging flow cytometry faces throughput limitations due to camera speed and sensitivity, hindering high-volume analysis.

Purpose of the Study:

To overcome the throughput limitations of imaging flow cytometry while maintaining image quality.
To introduce and detail the asymmetric-detection time-stretch optical microscopy (ATOM) as a solution for high-throughput cellular imaging.
To describe a protocol for establishing an ATOM system and its workflow for imaging flow cytometry.

Main Methods:

Utilized asymmetric-detection time-stretch optical microscopy (ATOM), an all-optical imaging technique relying on ultrafast broadband laser pulses.
Enhanced image contrast of unlabeled/unstained cells by accessing spectral phase-gradient information.
Developed a protocol covering optical frontend, data processing, and visualization backend for the ATOM system.

Main Results:

Demonstrated ATOM's capability for high-contrast, single-cell imaging with sub-cellular resolution at an unprecedented throughput of 100,000 cells/s.
Showcased ATOM's advantage in high-throughput measurements of single-cell morphology and texture for unlabeled cells.
Successfully applied the ATOM-based imaging flow cytometry workflow to human cells and micro-algae.

Conclusions:

ATOM is a powerful imaging flow cytometry platform that overcomes throughput limitations while preserving image quality.
ATOM enables biophysical phenotyping of cells through detailed morphology and texture analysis, complementing biochemical assays.
The described protocol facilitates the establishment and application of ATOM for diverse life science research, diagnostics, and monitoring.