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ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
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ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth.

Thurston Herricks1, Fred D Mast2, Song Li3

  • 1Institute for Systems Biology; therrick@systemsbiology.org.

Journal of Visualized Experiments : Jove
|July 18, 2017
PubMed
Summary

We developed a new method, One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY), to precisely measure microorganism growth across all phases. This technique reveals population heterogeneity, offering insights into genetic fitness and responses to environmental changes.

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Area of Science:

  • Microbiology
  • Genetics
  • Cell Biology

Background:

  • Microorganism growth phenotypes reflect genetic fitness and are categorized into lag, log, and stationary phases.
  • Each growth phase provides distinct insights into fitness under varying genetic and environmental conditions.
  • Quantitative, high-resolution measurement of all three growth phases is challenging.

Purpose of the Study:

  • To present a detailed method for characterizing all three growth phases of microorganisms on solid media.
  • To introduce the One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY) assay.
  • To enable high-resolution, quantitative analysis of microbial growth dynamics.

Main Methods:

  • The ODELAY assay utilizes time-lapse microscopy to quantify growth phenotypes of individual cells forming colonies on solid media.
  • This method allows direct observation of population heterogeneity within genetically identical cell populations.
  • The technique is applicable to any colony-forming microorganism observable via bright-field microscopy.

Main Results:

  • ODELAY provides detailed characterization of lag, log, and stationary growth phases.
  • The assay quantifies individual cell growth into colonies, revealing population heterogeneity.
  • Demonstrated applicability in yeast, with potential for broader microbial studies.

Conclusions:

  • ODELAY offers a novel approach for high-resolution, quantitative analysis of microbial growth.
  • The method facilitates the study of population heterogeneity, crucial for understanding genetic and epigenetic regulation.
  • ODELAY provides a unique perspective on cellular responses to genetic and environmental perturbations.