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Related Concept Videos

Confocal Fluorescence Microscopy01:16

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Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
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Related Experiment Video

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Multiplexing Focused Ultrasound Stimulation with Fluorescence Microscopy
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Electrically tunable fluidic lens imaging system for laparoscopic fluorescence-guided surgery.

Davide Volpi1, Iain D C Tullis1, Paul R Barber1

  • 1CR-UK/MRC Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, Oxford, OX3 7DQ, UK.

Biomedical Optics Express
|July 19, 2017
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Summary

This study introduces a new laparoscope camera coupler with a tunable fluidic lens for enhanced fluorescence-guided surgery. It improves focus control and detection sensitivity for near-infrared markers.

Keywords:
(120.3620) Lens system design(120.4820) Optical systems(170.0110) Imaging systems(170.2150) Endoscopic imaging(170.6280) Spectroscopy, fluorescence and luminescence

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Area of Science:

  • Medical Imaging
  • Optical Engineering
  • Surgical Technology

Background:

  • Fluorescence guidance using near-infrared (NIR) markers is increasingly important in laparoscopic surgery.
  • Current laparoscope technology has limitations in working distance and detection sensitivity for molecularly targeted probes.

Purpose of the Study:

  • To develop and evaluate a novel laparoscope camera coupler with programmable focus control.
  • To enhance optical performance for fluorescence-guided laparoscopic surgery, particularly in the NIR spectrum.

Main Methods:

  • Development of a laparoscope camera coupler featuring an electrically tunable fluidic lens.
  • Characterization of the coupler's achromatic performance across visible and NIR wavelengths.
  • Demonstration of optical performance in simulated and *in vivo* laparoscopic fluorescence-guided surgery.

Main Results:

  • The novel coupler offers programmable focus control and desirable achromatic performance from visible to NIR light.
  • It extends the lower working distance limit and improves detection sensitivity for fluorescence markers.
  • Successful *in vivo* demonstration using both tumor-specific molecular probes and nonspecific NIR dyes.

Conclusions:

  • The developed fluidic lens-based coupler significantly enhances optical performance in laparoscopic fluorescence-guided surgery.
  • This technology improves the utility of NIR markers for molecularly targeted imaging during minimally invasive procedures.
  • The coupler shows promise for advancing precision and efficacy in surgical interventions.