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Formation of the Platelet Plug01:22

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The platelet phase, the second stage of hemostasis, commences around 15-20 seconds after an injury. It follows and overlaps with the vascular phase, during which blood vessels constrict to minimize blood loss.
As the injured blood vessel contracts, endothelial cells undergo contraction, revealing collagen fibers in the basement membrane and underlying connective tissue. Furthermore, the plasma membrane of endothelial cells becomes adhesive, preparing the site for platelet adhesion. Platelets...
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Related Experiment Video

Updated: Feb 26, 2026

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
11:18

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice

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Evaluating platelet aggregation dynamics from laser speckle fluctuations.

Zeinab Hajjarian1, Diane M Tshikudi1, Seemantini K Nadkarni1

  • 1Wellman Center for Photomedicine, Massachusetts General Hospital and Harvard Medical School, 40 Blossom Street, Boston, MA 02114, USA.

Biomedical Optics Express
|July 19, 2017
PubMed
Summary

This study introduces a novel laser speckle method to rapidly assess platelet aggregation. The technique quantizes speckle fluctuations to determine aggregate size, aiding in diagnosing bleeding disorders and monitoring anti-platelet therapies.

Keywords:
(030.6140) Speckle(110.6150) Speckle imaging(170.1610) Clinical applications(170.4580) Optical diagnostics for medicine(290.0290) Scattering

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Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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Area of Science:

  • Biomedical Optics
  • Hematology
  • Nanotechnology

Background:

  • Platelets are crucial for hemostasis; impaired aggregation can cause bleeding or thrombosis.
  • Current methods for assessing platelet function can be time-consuming.
  • A rapid, accurate method for platelet aggregation analysis is needed.

Purpose of the Study:

  • To develop and validate a novel laser speckle-based approach for profiling platelet aggregation.
  • To assess the method's efficacy in quantifying aggregation dynamics and inhibition.

Main Methods:

  • Utilized laser speckle intensity fluctuations from platelet-rich plasma (PRP) drops.
  • Quantified speckle intensity autocorrelation (g) to deduce aggregate size.
  • Applied spatio-temporal speckle analysis to create 2D displacement maps and visualize aggregates.

Main Results:

  • Successfully evaluated polystyrene bead aggregation triggered by salt.
  • Demonstrated dose-dependent platelet aggregation and inhibition by adenosine diphosphate and clopidogrel in human PRP.
  • Visualized and quantified platelet aggregate foci and dynamics within minutes.

Conclusions:

  • The laser speckle method offers a rapid, minutes-long assessment of platelet aggregation.
  • This technique holds potential for diagnosing bleeding disorders.
  • It can also be used for monitoring the effectiveness of anti-platelet therapies.