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An interdigital array microelectrode aptasensor based on multi-walled carbon nanotubes for detection of tetracycline
Wenjie Hou1,2, Zhaoqiang Shi1,2, Yemin Guo3,4
1School of Agriculture and Food Engineering, Shandong University of Technology, Zibo, People's Republic of China.
Bioprocess and Biosystems Engineering
|July 19, 2017
Summary
A new impedance aptasensor detects tetracycline (TET) rapidly and selectively using modified microelectrodes. This sensor shows high sensitivity for tetracycline detection in milk samples.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Tetracycline (TET) is a widely used antibiotic.
- Accurate detection of TET is crucial for food safety and quality control.
- Existing detection methods can be time-consuming or lack sensitivity.
Purpose of the Study:
- To design a sensitive, selective, and fast impedance aptasensor for tetracycline detection.
- To utilize an interdigital array microelectrode (IDAM) for enhanced performance.
- To validate the aptasensor's efficacy in real-world samples like milk.
Main Methods:
- Modification of an IDAM with multi-walled carbon nanotubes (MWCNTs) for aptamer immobilization.
- Utilizing electrochemical impedance spectroscopy (EIS) for detection.
- Quantifying TET based on changes in electron transfer resistance (R et).
Main Results:
- The aptasensor demonstrated sensitive impedance changes upon TET binding.
- A significant increase in R et was observed with increasing TET concentration.
- A wide linear detection range from 10-9 to 10-3 M was achieved.
- The method was successfully applied to detect TET in milk samples.
Conclusions:
- The developed impedance aptasensor offers a sensitive, selective, and rapid method for TET detection.
- MWCNT modification enhances aptamer immobilization and sensor performance.
- The aptasensor shows potential for practical application in food analysis.
