Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

99.4K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
99.4K
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

775
Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
775
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

630
Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...
630
Genomics02:02

Genomics

41.1K
Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...
41.1K
RNA-seq03:21

RNA-seq

12.2K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
12.2K
Genome Annotation and Assembly03:36

Genome Annotation and Assembly

21.2K
The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.
21.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Interlaboratory controls: more than just a knowledge test].

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2016
Same author

[Detection and typing by molecular biology of human papillomavirus in genital samples].

Revista espanola de quimioterapia : publicacion oficial de la Sociedad Espanola de Quimioterapia·2006
See all related articles

Related Experiment Video

Updated: Feb 26, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

84.7K

[Microbiome and next generation sequencing].

A Suárez Moya1

  • 1Avelina Suárez Moya, Hospital Clínico San Carlos. Plaza de Cristo Rey s/n, 28040 Madrid, Spain. avelina.suarez@salud.madrid.org.

Revista Espanola De Quimioterapia : Publicacion Oficial De La Sociedad Espanola De Quimioterapia
|July 19, 2017
PubMed
Summary

The human microbiome, a complex ecosystem of microorganisms, is crucial for health. Advanced sequencing technologies have revolutionized our understanding of its role in human well-being and disease.

More Related Videos

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

31.3K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.7K

Related Experiment Videos

Last Updated: Feb 26, 2026

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
10:24

Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons

Published on: August 29, 2014

84.7K
Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
11:22

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

31.3K
Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing
08:05

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

20.7K

Area of Science:

  • Microbiology
  • Genomics
  • Human Health

Background:

  • The human microbiome is an internal ecosystem vital for health, interacting with the immune system to protect against pathogens.
  • Traditional bacteriological culture methods are insufficient for studying most environmental microorganisms.
  • Metagenomics offers a culture-independent approach to identify microorganisms via their genomic fingerprint.

Purpose of the Study:

  • To highlight the advancements in sequencing technologies for human microbiome research.
  • To emphasize the impact of these technologies on understanding microbial taxonomy and function.
  • To underscore the revolution in microbiome research and its implications for human health and disease.

Main Methods:

  • Utilizing metagenomic approaches for microbial identification through genomic sequencing.
  • Employing 16S rRNA sequencing as a universal bacterial identification target.
  • Leveraging second and third-generation high-throughput sequencing technologies for massive data generation.
  • Applying bioinformatics tools for processing and analyzing large-scale sequencing data.

Main Results:

  • Metagenomic techniques, particularly advanced sequencing, allow for detailed identification of microorganisms.
  • Massive sequencing and bioinformatics have enabled unprecedented insights into microbial taxonomy and function.
  • The study of the human microbiome has reached a new level of detail and understanding.

Conclusions:

  • Modern sequencing technologies have revolutionized human microbiome research.
  • This advancement provides a deeper understanding of the microbiome's role in human health and disease.
  • The findings signify a paradigm shift in microbiology and its clinical applications.