Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

5.0K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
5.0K
Atomic Spectroscopy: Absorption, Emission, and Fluorescence01:23

Atomic Spectroscopy: Absorption, Emission, and Fluorescence

3.0K
Atomic spectroscopy is a vital tool in elemental analysis, both qualitatively and quantitatively. It can be broadly divided into optical spectroscopy, mass spectroscopy, and X-ray spectroscopy methods. The optical spectroscopic methods are atomic absorption spectroscopy (AAS), atomic emission spectroscopy (AES), and atomic fluorescence spectroscopy (AFS). The first step in all three methods is atomization, where the solid, liquid, or solution-phase samples are converted into gas-phase atoms and...
3.0K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

3.2K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
3.2K
Atomic Fluorescence Spectroscopy01:29

Atomic Fluorescence Spectroscopy

1.0K
Atomic fluorescence spectroscopy (AFS) is an analytical technique that involves the electronic transitions of atoms in a flame, furnace, or plasma being excited by electromagnetic (EM) radiation. When these atoms absorb energy, they become excited and subsequently release energy as they return to their original state. This emitted light, or "fluorescence," is observed at a right angle to the incident beam. Both absorption and emission processes transpire at distinct wavelengths, which...
1.0K
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.7K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exact integrated equations to describe diffusion kinetics.

Soft matter·2026
Same author

Diffusion kinetics of volatile organic compounds monitored by nanohole surface plasmonics.

The Analyst·2026
Same author

Mineralogical Analysis of Solid-Sample Flame Emission Spectra by Machine Learning.

Analytical chemistry·2024
Same author

Coaxial burner system for solid-sample flame emission spectroscopy.

Analytical methods : advancing methods and applications·2024
Same author

Dolutegravir quantification in wistar rat tissues following chronic administration.

Heliyon·2023
Same author

Accelerated size-focusing light activated synthesis of atomically precise fluorescent Au<sub>22</sub>(Lys-Cys-Lys)<sub>16</sub> clusters.

Nanoscale·2023
Same journal

The ACS at 150: The History of Analytical Chemistry Publications and a Century of Progress.

Analytical chemistry·2026
Same journal

Machine Learning-Enabled Image Analysis of Complex Chemical Mixtures: Synthetic Urine Droplets as a Test System.

Analytical chemistry·2026
Same journal

H<sub>2</sub>O<sub>2</sub>/Viscosity Tandem-Locked Fluorescent Probes Based on an In Situ Fluorophore Synthesis Strategy for Colitis Imaging and Diagnosis.

Analytical chemistry·2026
Same journal

TopoStitcher: A Geometric-Topological Structure-Guided Stitching Framework for Single-Molecule Localization Microscopy.

Analytical chemistry·2026
Same journal

Noninvasive SERS Immunosensing of Tyrosinase for Melanoma Monitoring via Microneedle Sampling Integrated with Satellite-Structured Bifunctional Nanozymes.

Analytical chemistry·2026
Same journal

Label-Free Electrochemical CRISPR Platform Gated by Allosteric Transcription Factors for Ultrasensitive Small-Molecule Detection.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 26, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.5K

Hadamard-Transform Fluorescence Excitation-Emission-Matrix Spectroscopy.

N L P Andrews1, T Ferguson1, A M M Rangaswamy1

  • 1Department of Chemistry, Queen's University , Kingston, Ontario K7L 3N6, Canada.

Analytical Chemistry
|July 19, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a novel fluorescence spectrometer that significantly accelerates data acquisition. This advancement enables faster analysis of complex samples, improving kinetic and chemical reaction studies.

More Related Videos

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

558
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K

Related Experiment Videos

Last Updated: Feb 26, 2026

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
07:34

Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

Published on: August 22, 2019

8.5K
A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

558
Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
12:51

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Published on: December 9, 2013

9.4K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Physical Chemistry

Background:

  • Conventional fluorescence excitation-emission-matrix (EEM) spectroscopy can be limited by slow data acquisition rates.
  • High-throughput analysis of dynamic fluorescence processes requires faster spectroscopic methods.

Purpose of the Study:

  • To develop and demonstrate a fluorescence EEM spectrometer with significantly enhanced data acquisition speeds.
  • To validate the performance of the new instrument by studying chemical kinetics and transformations.

Main Methods:

  • Utilized a white light emitting diode (LED) source and a digital micromirror array (DMA) for encoded excitation light.
  • Employed binary n-size Walsh functions for light encoding and inverse Hadamard transformation for EEM reconstruction.
  • Integrated a conventional array spectrometer for fluorescence detection.

Main Results:

  • Achieved data acquisition rates significantly higher than conventional EEM spectrometers, with potential increases of up to [n(n + 1)]/2-fold.
  • Demonstrated spectral acquisition rates exceeding two spectra per second.
  • Successfully monitored the temperature-dependent fluorescence kinetics of rhodamine B and the demetalation of chlorophyll-a to pheophytin-a.

Conclusions:

  • The developed fluorescence EEM spectrometer offers a substantial improvement in speed for acquiring complex spectral data.
  • This high-speed capability is well-suited for investigating rapid fluorescence phenomena and dynamic chemical processes.
  • The instrument provides a powerful tool for advanced analytical applications in chemistry and related fields.