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Hadamard-Transform Fluorescence Excitation-Emission-Matrix Spectroscopy
N L P Andrews1, T Ferguson1, A M M Rangaswamy1
1Department of Chemistry, Queen's University , Kingston, Ontario K7L 3N6, Canada.
Analytical Chemistry
|July 19, 2017
Summary
This study introduces a novel fluorescence spectrometer that significantly accelerates data acquisition. This advancement enables faster analysis of complex samples, improving kinetic and chemical reaction studies.
Area of Science:
- Analytical Chemistry
- Spectroscopy
- Physical Chemistry
Background:
- Conventional fluorescence excitation-emission-matrix (EEM) spectroscopy can be limited by slow data acquisition rates.
- High-throughput analysis of dynamic fluorescence processes requires faster spectroscopic methods.
Purpose of the Study:
- To develop and demonstrate a fluorescence EEM spectrometer with significantly enhanced data acquisition speeds.
- To validate the performance of the new instrument by studying chemical kinetics and transformations.
Main Methods:
- Utilized a white light emitting diode (LED) source and a digital micromirror array (DMA) for encoded excitation light.
- Employed binary n-size Walsh functions for light encoding and inverse Hadamard transformation for EEM reconstruction.
- Integrated a conventional array spectrometer for fluorescence detection.
Main Results:
- Achieved data acquisition rates significantly higher than conventional EEM spectrometers, with potential increases of up to [n(n + 1)]/2-fold.
- Demonstrated spectral acquisition rates exceeding two spectra per second.
- Successfully monitored the temperature-dependent fluorescence kinetics of rhodamine B and the demetalation of chlorophyll-a to pheophytin-a.
Conclusions:
- The developed fluorescence EEM spectrometer offers a substantial improvement in speed for acquiring complex spectral data.
- This high-speed capability is well-suited for investigating rapid fluorescence phenomena and dynamic chemical processes.
- The instrument provides a powerful tool for advanced analytical applications in chemistry and related fields.
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