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TRIM59 is a key regulator of growth and migration inrenal cell carcinoma

S-H Hu1, M-J Zhao2, W-X Wang3

  • 1Department of Clinical Laboratory, Affiliated Hospital of Dali University, Dali, China.

Cellular and Molecular Biology (Noisy-Le-Grand, France)
|July 19, 2017
PubMed

Insights

Tripartite motif 59 (TRIM59) promotes renal cell carcinoma (RCC) progression by enhancing cell growth and migration. Knocking down TRIM59 significantly inhibited tumor growth in vitro and in vivo, suggesting its potential as a therapeutic target for RCC.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Renal cell carcinoma (RCC) is a prevalent malignancy with a high metastatic rate.
  • Tripartite motif (TRIM) family proteins are implicated in human tumorigenesis.
  • The specific role of TRIM59 in RCC progression requires further investigation.

Purpose of the Study:

  • To investigate the role of TRIM59 in the cell growth and migration of renal cell carcinoma.
  • To evaluate the potential of TRIM59 as a therapeutic target for RCC.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) to assess TRIM59 mRNA expression in RCC tissues.
  • Lentivirus-based short hairpin RNA (shRNA) for TRIM59 knockdown in RCC cell lines.
  • In vitro assays (MTT, colony formation, Transwell) and in vivo mouse xenograft models to evaluate proliferation, migration, and invasion.

Main Results:

  • TRIM59 mRNA levels were significantly upregulated (2-fold) in cancerous RCC tissues compared to non-cancerous tissues.
  • TRIM59 knockdown in 786-O cells reduced cell proliferation, colony formation, and tumor growth in vivo.
  • TRIM59 depletion suppressed cell migration by 68% and invasion by 50% in 786-O cells.

Conclusions:

  • TRIM59 acts as a pro-oncogenic protein, promoting the progression of renal cell carcinoma.
  • Targeting TRIM59 through knockdown presents a promising strategy for the early detection and treatment of RCC.
Keywords:
GrowthInvasionMigrationRenal cell carcinoma.TRIM59

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