Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Master Transcription Regulators02:23

Master Transcription Regulators

7.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.9K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.4K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.4K
The Eukaryotic Promoter Region02:40

The Eukaryotic Promoter Region

19.2K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences.  The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
19.2K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

17.0K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
17.0K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.7K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

12.0K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
12.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative Outcomes of Fine Needle Aspiration and Core Needle Biopsy for Parotid Masses.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2026
Same author

P2-HNF4α alters linoleic acid metabolism and mitigates soybean oil-induced obesity: role for oxylipins.

Journal of lipid research·2025
Same author

Poly(carboxybetaine) lipids enhance mRNA therapeutics efficacy and reduce their immunogenicity.

Nature materials·2025
Same author

Mixed-Methods Approach: Impact of Clinical Consenter Diversity on Clinical Trials Enrollment.

Cancers·2025
Same author

Parotidectomy Trends Toward Outpatient for Benign Disease.

Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery·2024
Same author

Patient-Initiated Communication After Parotidectomy.

The Laryngoscope·2024

Related Experiment Video

Updated: Feb 26, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.3K

Sequence-specific DNA binding by MYC/MAX to low-affinity non-E-box motifs.

Michael Allevato1, Eugene Bolotin2, Mark Grossman1

  • 1Department of Biochemistry, University of California Riverside, Riverside, California, United States of America.

Plos One
|July 19, 2017
PubMed
Summary

The MYC oncoprotein binds diverse DNA motifs with varying affinities, not just canonical E-boxes. Elevated MYC levels influence binding in a dose-dependent manner, impacting tumor transcriptome selectivity.

More Related Videos

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.6K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.7K

Related Experiment Videos

Last Updated: Feb 26, 2026

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
14:47

Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry

Published on: May 17, 2016

10.3K
Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos
11:02

Chromatin Immunoprecipitation Assay for Tissue-specific Genes using Early-stage Mouse Embryos

Published on: April 29, 2011

18.6K
CD Spectroscopy to Study DNA-Protein Interactions
06:48

CD Spectroscopy to Study DNA-Protein Interactions

Published on: February 10, 2022

7.7K

Area of Science:

  • Molecular Biology
  • Genomics
  • Cancer Biology

Background:

  • The MYC oncoprotein is a transcription factor crucial for cell growth and proliferation.
  • MYC functions as a heterodimer with MAX, binding to Enhancer box (E-box) DNA sequences.
  • Previous studies suggested MYC:MAX binds DNA non-specifically, recognizing E-box variants.

Purpose of the Study:

  • To identify additional non-canonical DNA binding sites for MYC:MAX.
  • To characterize the binding affinity and sequence specificity of MYC:MAX.
  • To understand the impact of MYC levels on DNA binding in vivo.

Main Methods:

  • High-throughput in vitro protein-binding microarrays.
  • Electrophoretic mobility-shift assays (EMSAs).
  • Bioinformatic analyses of MYC-bound genomic loci in vivo.

Main Results:

  • Identified hexameric motifs preferentially bound by MYC/MAX, including the low-affinity sequence AACGTT.
  • Found that 87% of MYC-bound genomic sites contain top MYC:MAX-bound motifs.
  • Demonstrated that high MYC/MAX concentrations are required for binding to low-affinity sites, and elevated MYC levels preferentially increase occupancy at these sites.

Conclusions:

  • MYC binds diverse DNA motifs with a broad range of affinities in a sequence-specific and dose-dependent manner.
  • MYC overexpression may have more selective effects on the tumor transcriptome than previously assumed.
  • These findings refine our understanding of MYC's transcriptional regulatory network in cancer.