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Beta-lactamase database (BLDB) - structure and function.

Thierry Naas1, Saoussen Oueslati1, Rémy A Bonnin1

  • 1a Service de Bactériologie-Hygiène, Hôpital de Bicêtre, AP-HP, EA7361 , Université et Faculté de Médecine Paris-Sud, LabEx LERMIT , Le Kremlin-Bicêtre , France.

Journal of Enzyme Inhibition and Medicinal Chemistry
|July 20, 2017
PubMed
Summary
This summary is machine-generated.

The Beta-Lactamase Database (BLDB) offers curated structural and functional data on beta-lactamase enzymes, crucial for understanding and combating antibiotic resistance. This public resource aids in tracking the evolution of resistance through advanced search capabilities.

Keywords:
Databaseantibiotic resistancebeta-lactamasehydrolytic profilemutant

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Structural Biology

Background:

  • Beta-lactamase enzymes are critical in the development of antibiotic resistance.
  • Understanding the structure-function relationship of these enzymes is essential for developing new therapeutic strategies.

Purpose of the Study:

  • To present a comprehensive, manually curated public resource for beta-lactamase enzymes.
  • To provide up-to-date structural, functional, and kinetic information on beta-lactamases.
  • To facilitate the surveillance of antibiotic resistance evolution.

Main Methods:

  • Manual curation of literature data on beta-lactamases.
  • Classification of enzymes by class (A, B, C, D), family, and subfamily.
  • Inclusion of all available three-dimensional structures from the Protein Data Bank.
  • Characterization of enzyme mutants and hydrolytic profiles (kinetics).
  • Integration of protein- and nucleotide-based BLAST search functionalities.

Main Results:

  • The Beta-Lactamase Database (BLDB) is established as a central repository for beta-lactamase information.
  • BLDB integrates enzyme classification, 3D structures, mutant data, and kinetic profiles.
  • The database supports BLAST searches for efficient data retrieval and analysis.

Conclusions:

  • BLDB provides a foundational resource for understanding beta-lactamase structure-function relationships.
  • The database is a valuable tool for monitoring the evolution of antibiotic resistance.
  • BLDB is freely accessible online, supporting modern web browsers without registration.