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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: Feb 26, 2026

Author Spotlight: Functional Site-Directed Fluorometry in Native Cells to Study Skeletal Muscle Excitability

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Using fluorescence to understand β subunit-Na_V channel interactions

Rene Barro-Soria¹, Sara I Liin², H Peter Larsson³

¹Department of Physiology and Biophysics, Miller School of Medicine, University of Miami, Miami, FL rbarro@med.miami.edu.

The Journal of General Physiology

|July 20, 2017

Summary

No abstract available in PubMed .

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