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Updated: Feb 26, 2026

Fibroblast Derived Human Engineered Connective Tissue for Screening Applications
Published on: August 20, 2021
Mimicking Paracrine TGFβ1 Signals during Myofibroblast Differentiation in 3D Collagen Networks
Michael Ansorge1, Jiranuwat Sapudom1, Marina Chkolnikov1
1Universität Leipzig, Institute of Biochemistry, Johannisallee 21/23, 04103, Leipzig, Germany.
Abstract:
TGFβ1 is a key regulator for induction of tissue remodeling after dermal wounding. We present a model of paracrine delivery of TGFβ1 for differentiation of dermal fibroblasts based on a fibrillar 3D collagen matrix and embedded TGFβ1 releasing microparticles. We found differentiation into myofibroblasts was achieved in a TGFβ1 dependent manner at much lower doses than systemic delivery. This effect is accounted to the slow and sustained TGFβ1 release mimicking paracrine cell signals.
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