The effects of ethane dimethane sulphonate (EDS) on bilaterally cryptorchid rat testes

A single dose of ethane dimethane sulphonate (EDS) given to adult male rats has a specific destructive effects on Leydig cells, which are removed from the intertubular area by macrophages. This is associated with a decrease in testosterone (T) and a rise in serum FSH and LH for 21-28 days after EDS. Recovery of Leydig cells occurs from connective tissue precursors. This study uses the bilaterally cryptorchid rat to investigate the influence of local factors from the seminiferous tubules on the restoration of Leydig cell morphology and function. Morphometric data of intertubular tissue, hCG binding, serum T, FSH and LH levels all indicate that the Leydig cell population in cryptorchid testes is more rapidly restored from connective tissue cells when compared to the normal situation. The precise mechanism for this faster recovery will require further study.