Releasing characteristics of anthocyanins extract in pectin-whey protein complex microcapsules coated with zein

This study investigated pectin-based capsules as delivery systems for purple rice bran anthocyanin extract (AE) during exposure to simulated gastrointestinal conditions. Four different capsules loaded with AE were prepared by ionotropic gelation/extrusion, including (1) pectin capsules (PE), (2) pectin capsules coated with zein (PE/ZE), (3) pectin-whey protein isolate complex capsules (PE + WP), and (4) pectin-whey protein isolate complex capsules coated with zein (PE + WP/ZE). CaCl₂ in an ethanol solution with or without zein was used as a crosslinking solution. Swelling and release characteristics of all capsules under simulated gastric fluid at pH 1.2 (SGF) and simulated intestinal fluid at pH 6.8 (SIF) for 120 and 180 min, respectively, were examined. PE + WP, PE + WP/ZE, and PE/ZE capsules had higher encapsulation efficiency than PE capsules. After incubation, PE + WP/ZE and PE capsules had the lowest swelling ratio in SGF and SIF, respectively. PE + WP/ZE capsules had the lowest AE release in SGF, while PE capsules had the highest. Both PE + WP and PE + WP/ZE capsules had significantly lower AE release in SIF than PE and PE/ZE capsules. The study demonstrated that PE + WP and PE + WP/ZE capsules have potential to function as a slow release delivery system for AE.