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Related Experiment Video

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Application of Optical Coherence Tomography to a Mouse Model of Retinopathy
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Whole mouse brain imaging using optical coherence tomography: reconstruction, normalization, segmentation, and

Joël Lefebvre1, Alexandre Castonguay1, Philippe Pouliot1,2

  • 1École Polytechnique de Montréal, Montréal, Québec, Canada.

Neurophotonics
|July 20, 2017
PubMed
Summary

Researchers developed a novel automated histology technique using optical coherence tomography (OCT) to image whole mouse brains. This method enables detailed, multimodal investigations of brain structure and tissue properties.

Keywords:
brain normalizationdiffusion MRIimage reconstructionmassive histologyoptical coherence tomographytissue segmentation

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Area of Science:

  • Neuroscience
  • Biomedical Engineering
  • Histology

Background:

  • Automated histology and optical coherence tomography (OCT) are advancing small animal brain research.
  • Imaging whole mouse brains at high resolution presents significant data processing challenges.

Purpose of the Study:

  • To develop and validate techniques for reconstructing and segmenting large datasets from OCT imaging of whole mouse brains.
  • To investigate the origins of OCT contrast in brain tissue by correlating optical reflectivity with microstructural properties.

Main Methods:

  • An automated massive histology setup coupled with an OCT microscope was used to image whole mouse brains.
  • Techniques for volumetric tile reconstruction and [Formula: see text]-tissue segmentation were employed.
  • Diffusion MRI (dMRI) was used to coregister with OCT data for multimodal comparison.

Main Results:

  • Average mouse brain templates for OCT reflectivity and attenuation contrasts were generated.
  • Optical reflectivity was found to vary with fiber orientation, density, and complexity.
  • Successful coregistration of OCT and dMRI data was achieved, validating the technique.

Conclusions:

  • The developed massive histology technique provides a powerful platform for cross-sectional, multimodal investigations of small animal brains.
  • This approach facilitates detailed analysis of brain tissue properties and their relationship to imaging contrasts.
  • The study highlights the potential for large-scale, multisubject investigations using OCT-based histology.