Suppressive effect of indazole adenine dinucleotides on proliferation of normal and malignant cells

The suppressive effects of newly synthesized NAD-analogs, indazole adenine dinucleotides (IAD), on the cellular proliferation of normal and L5178Y cells were investigated in connection with their inhibitory activity on inosine 5'-monophosphate dehydrogenase (IMPD). All the dinucleotides, except compounds VIII and IX, were observed to show a certain extent of suppression at a concentration of 100 micrograms/ml. In particular, some compounds (II, VII, X and XI) among them showed a strong suppression (70-90%) in the leukemic cell system. The suppression seemed to be roughly correlative with the degree of IMPD inhibition of the dinucleotide.