Lanthanide-Labeled Immunochromatographic Strip Assay for the On-Site Identification of Ancient Silk

The on-site identification of ancient silks has long been a key challenge in archeology. Therefore, a rapid, cost-effective, sensitive analytical approach is highly desirable. In this paper, a lanthanide-labeled immunochromatographic strip which is suitable for the on-site identification of ancient silks is described. Compared with the conventional colloidal gold-based immunochromatographic strip, this strip shows much higher analytical sensitivity and better quantitative discrimination. The limit of detection (LOD) of the strip for silk fibroin (SF) was calculated as 8.09 ng/mL, approximately 185 times lower than that of the colloidal gold-based immunochromatographic strip. No cross-reactions with other possible interfering antigens were observed. Moreover, the strip also shows good reproducibility, with a mean recovery of 94.15-102.55% and coefficient of variation of 5.22-17.57% in the repeated tests. Based on the advantages of portability and cost-effectiveness, as well as sensitivity, specificity, and reproducibility, the lanthanide-labeled immunochromatographic strip is a promising tool for on-site detection of ancient relics in archeological fieldwork.