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Related Concept Videos

Meiosis II01:57

Meiosis II

209.4K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
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Meiosis II02:02

Meiosis II

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Meiosis II entails cell division and segregation of the sister chromatids, resulting in the production of four unique haploid gametes. The steps for meiosis II are similar to mitosis, except that meiosis II occurs in haploid cells, whereas mitosis occurs in diploid cells.
The timing and cell division patterns of meiosis differ between males and females. In male meiosis, the centrosomes are part of the formation of the meiotic spindle. However, in oocytes, including that of humans, Drosophila,...
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Meiosis I03:09

Meiosis I

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Meiosis is the division of a diploid cell into haploid cells forming sperm and eggs in animals through differentiation. Meiosis I is the first stage of meiosis, where the genetic recombination of homologous chromosomes and the reduction of the ploidy level by half occurs.
Prophase I is the most extended and complex step of meiosis I characterized by synapsis, chromosome pairing, and recombination of the homologous chromosomes. This process is facilitated by a proteinaceous structure called the...
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Spermatogenesis01:41

Spermatogenesis

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Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermatogenesis01:22

Spermatogenesis

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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Related Experiment Video

Updated: Feb 26, 2026

Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry
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Functional Assessment of Kinesin-7 CENP-E in Spermatocytes Using In Vivo Inhibition, Immunofluorescence and Flow Cytometry

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Emi2 Is Essential for Mouse Spermatogenesis.

Lakshmi Gopinathan1, Radoslaw Szmyd2, Diana Low1

  • 1Institute of Molecular and Cell Biology (IMCB), A(∗)STAR (Agency for Science, Technology and Research), 61 Biopolis Drive, Proteos #3-09, Singapore 138673, Republic of Singapore.

Cell Reports
|July 21, 2017
PubMed
Summary
This summary is machine-generated.

Emi2 is crucial for meiosis in both oocytes and sperm. Emi2 knockout mice are sterile, showing Emi2 is essential for male meiosis I progression and has potential post-meiotic roles.

Keywords:
APC/CCdk1Emi2diploteneknockout micemeiosisovaryphosphorylationspermatocytesspermatogenesis

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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
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A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
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Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
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Area of Science:

  • Reproductive Biology
  • Cell Cycle Regulation
  • Molecular Genetics

Background:

  • Emi2, an APC/C inhibitor, is known for its role in oocyte meiotic arrest.
  • Emi2 is highly expressed in the testis, suggesting important functions in male gametogenesis.

Purpose of the Study:

  • To investigate the in vivo functions of Emi2 in the testis.
  • To elucidate the role of Emi2 in male meiosis and potential post-meiotic functions.

Main Methods:

  • Generation of Emi2 knockout mice.
  • Analysis of meiotic progression in spermatocytes.
  • Assessment of Cdk1 activity in spermatocytes.

Main Results:

  • Emi2 knockout mice are viable but sterile.
  • Emi2 is essential for meiosis I progression in spermatocytes, with arrest at early diplotene of prophase I.
  • Spermatocyte arrest is linked to decreased Cdk1 activity and partially rescued by elevated Cdk1.
  • Emi2 is expressed in spermatids and sperm, indicating potential post-meiotic roles.

Conclusions:

  • Emi2 is essential for male fertility and plays a critical role in meiosis I progression in spermatocytes.
  • Emi2's function extends beyond oocyte meiotic arrest, highlighting its broader importance in mammalian reproduction.
  • Further research is warranted to explore the post-meiotic functions of Emi2.