Effects of calcium on the performance, bacterial population and microbial metabolism of a denitrifying phosphorus
Hongliang Dai1, Yifeng Wu1, Lihong Peng1
1School of Energy and Environment, Southeast University, No. 2 Sipailou Road, Nanjing 210096, China; ERC Taihu Lake Water Environment (Wuxi), No. 99 Linghu Road, Wuxi 214135, China.
Abstract:
A sequencing batch reactor was operated to study the effects of influent Ca2+ on the efficiency, bacterial population, and microbial metabolism of denitrifying phosphorus removal system. Results showed that high Ca2+ loading (≥80mg/L) significantly inhibited the performance of simultaneous nitrogen and phosphorus removal. The abundance of phosphorus removal-related organisms (Dechloromonas and Candidatus Accumulibacter) decreased with increasing Ca2+ concentration from 20 to 140mg/L, while the abundance of glycogen-accumulating organisms and other bacteria increased. Metabolomic analyses revealed that the metabolic profiles of microbial community were also affected by high influent Ca2+ concentrations. 3-Hydroxybutyrate, acetate, alanine, and glutamate were the main differentiated metabolites in the system. An accumulation of amino acids and a reduction of nucleotides and amines were important response to high Ca2+ loading. Long-term Ca2+ loading had a reversible effect on the denitrifying phosphorus removal system as it could revive after a 50-day recovery process.
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