Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Super-resolution Fluorescence Microscopy

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Widefield pump-probe microscopy with coherent background subtraction by angle-compensated temporal shearing.

Optics express·2026

Same author

Subthreshold membrane depolarization powerfully engages intracellular calcium dynamics in the brain.

bioRxiv : the preprint server for biology·2026

Same author

Implications of temporal sampling in voltage imaging microscopy.

ArXiv·2026

Same author

Relative phase of membrane potential theta oscillations between individual hippocampal neurons code space.

bioRxiv : the preprint server for biology·2025

Same author

Superresolution imaging of live samples by centroid reassignment microscopy.

bioRxiv : the preprint server for biology·2025

Same author

Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy.

Nature communications·2025

Same journal

Serum vitamin D level and its association with vertigo frequency and severity in Meniere disease.

Scientific reports·2026

Same journal

PFA-Net: a physics-informed feature enhancement and attention network for interpretable bearing fault diagnosis under strong noise.

Scientific reports·2026

Same journal

Circulating inflammatory, redox, and apoptosis-related alterations in drug-naive idiopathic pulmonary fibrosis: an exploratory case-control study.

Scientific reports·2026

Same journal

A baseline-oriented dynamic aggregation approach for demand-side heterogeneous controllable resources.

Scientific reports·2026

Same journal

Temporal precision and accuracy in schizophrenia: an exploratory study.

Scientific reports·2026

Same journal

Prefrontal EEG spectral and nonlinear signatures of subthreshold depression during resting state and affectively valenced picture/video viewing: a participant-level analysis.

Scientific reports·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Feb 26, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

Neuronal imaging with ultrahigh dynamic range multiphoton microscopy.

Ruohui Yang¹, Timothy D Weber¹, Ellen D Witkowski²

¹Boston University Department of Biomedical Engineering, 44 Cummington Mall, Boston, MA, 02215, USA.

Scientific Reports

|July 21, 2017

Summary

This summary is machine-generated.

This study introduces a digital add-on to improve multiphoton microscope dynamic range, preventing signal loss and photodamage. The technique enhances imaging of weak features and unpredictable sample strengths without hardware changes.

More Related Videos

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Published on: February 5, 2022

Related Experiment Videos

Last Updated: Feb 26, 2026

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Published on: August 23, 2022

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Author Spotlight: Comparative Imaging of Neural Activity in Awake and Freely Moving States

Published on: January 19, 2024

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

Published on: February 5, 2022

Area of Science:

Biomedical Optics
Microscopy Technology
Neuroscience Imaging

Background:

Multiphoton microscopy is limited by a narrow dynamic range, hindering detection of faint signals alongside strong ones.
Unpredictable fluctuations in sample signal strength pose challenges for consistent imaging.
Existing limitations can lead to loss of critical data and potential sample photodamage.

Purpose of the Study:

To develop a technique for significantly enhancing the dynamic range of multiphoton microscopes.
To mitigate photodamage during multiphoton imaging.
To enable detection of weak sample features and handle signal bursts without data loss.

Main Methods:

A digital electronic add-on module was designed for multiphoton microscopes.
The system implements real-time negative feedback to control laser power delivered to the sample.
A logarithmic representation of sample signal strength was employed to achieve ultrahigh dynamic range.

Main Results:

The add-on successfully expanded the dynamic range of multiphoton microscopy.
The technique effectively regulated laser power, minimizing photodamage.
No modifications to the existing microscope hardware were necessary, ensuring broad compatibility.
Demonstrated improved performance in both structural and in-vivo functional mouse brain imaging.

Conclusions:

The digital add-on offers a practical solution to overcome the dynamic range limitations of multiphoton microscopy.
This technology enhances imaging capabilities for diverse biological samples, including live mouse brains.
The approach is compatible with commercial instruments, facilitating widespread adoption.