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Interference and Diffraction02:18

Interference and Diffraction

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Interference is a characteristic phenomenon exhibited by waves. When two electromagnetic waves interact with their peaks and troughs coinciding, a resulting wave with enhanced amplitude is produced. This is known as constructive interference. In this case, the two waves interacting are in phase with each other.
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IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations01:08

IR Spectrum Peak Splitting: Symmetric vs Asymmetric Vibrations

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Identical bonds within a polyatomic group can stretch symmetrically (in-phase) or asymmetrically (out-of-phase). Similar to hydrogen bonding, these vibrations also influence the shape of the IR peak. Generally, asymmetric stretching frequencies are higher than symmetric stretching frequencies. For example, primary amines exhibit two distinct IR peaks between 3300–3500 cm−1 corresponding to the symmetric and asymmetric N-H stretching, while secondary amines exhibit a single...
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Relative Motion Analysis using Rotating Axes01:25

Relative Motion Analysis using Rotating Axes

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Consider a component AB undergoing a linear motion. Along with a linear motion, point B also rotates around point A. To comprehend this complex movement, position vectors for both points A and B are established using a stationary reference frame.
However, to express the relative position of point B relative to point A, an additional frame of reference, denoted as x'y', is necessary. This additional frame not only translates but also rotates relative to the fixed frame, making it...
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Spherical Coordinates01:23

Spherical Coordinates

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Spherical coordinate systems are preferred over Cartesian, polar, or cylindrical coordinates for systems with spherical symmetry. For example, to describe the surface of a sphere, Cartesian coordinates require all three coordinates. On the other hand, the spherical coordinate system requires only one parameter: the sphere's radius. As a result, the complicated mathematical calculations become simple. Spherical coordinates are used in science and engineering applications like electric and...
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IR Frequency Region: X–H Stretching01:24

IR Frequency Region: X–H Stretching

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In IR spectroscopy, signals produced by the X−H bonds (such as C−H, O−H, or N−H) can be observed in the frequency range of  2700–4000 cm–1. The C−H stretching vibration forms sharp bands in the region 2850–3000 cm–1. The presence of the O−H stretching vibration leads to the forming of an absorption band in the frequency range 3650–3200 cm−1. At the same time, N−H stretching can be confirmed by absorption bands in...
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Radius of Gyration of an Area01:12

Radius of Gyration of an Area

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The second moment of area, also known as the moment of inertia of area, is a crucial factor in understanding an object's resistance against bending deformation, or stiffness. To accurately estimate the second moment of area along any axis, one needs to concentrate all areas associated with that object into a thin strip, which should be placed parallel to that particular axis.
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Related Experiment Video

Updated: Feb 26, 2026

A Multimodal Wide-Field Fourier-Transform Raman Microscope
06:48

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

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Interferometric rotating point spread function.

Wei Wang1, Guohai Situ2,3

  • 1Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai, 201800, China.

Scientific Reports
|July 21, 2017
PubMed
Summary
This summary is machine-generated.

We developed an interferometric double helix point spread function (PSF) for super-resolution microscopy. This new method significantly expands the imaging depth range for precise molecular localization.

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Area of Science:

  • Optical microscopy
  • Super-resolution imaging
  • Biophysics

Background:

  • Rotating point spread functions (PSFs), like the double helix (DH) PSF, are crucial for super-resolution imaging due to their extended working depth.
  • Existing interferometric localization-based super-resolution methods have limitations in their imaging range.

Purpose of the Study:

  • To propose a novel interferometric DH PSF (iDH PSF) for enhanced super-resolution microscopy.
  • To improve the axial super-resolution by transferring it to azimuthal rotation.
  • To achieve a significantly larger imaging range compared to existing methods.

Main Methods:

  • Implementation of an iDH PSF using two opposed objective lenses, inspired by 4Pi microscopy.
  • Characterization of the iDH PSF's performance in terms of resolution and imaging depth.

Main Results:

  • The proposed iDH PSF successfully transfers axial super-resolution to azimuthal rotation.
  • An iDH PSF was designed with an imaging range of 3 μm.
  • This imaging range is approximately three times greater than that achieved by other interferometric localization-based super-resolution techniques.

Conclusions:

  • The iDH PSF offers a substantial advancement in super-resolution microscopy.
  • This technique enables deeper imaging with high precision, facilitating better visualization of biological structures.