Related Experiment Video

Updated: Oct 27, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.2K

Direct repeat unit (dru) typing and antimicrobial resistance of methicillin-resistant Staphylococcus pseudintermedius

Matthew E Saab1, J Scott Weese1, J T McClure1

  • 1Diagnostic Services, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island (Saab); Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, 550 University Avenue, Charlottetown, Prince Edward Island (Saab, McClure); Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario (Weese).

Canadian Journal of Veterinary Research = Revue Canadienne De Recherche Veterinaire
|July 21, 2017
PubMed

Insights

Methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Canadian dogs was characterized using direct repeat unit (dru) typing. Atlantic Canada showed a unique distribution of MRSP strains compared to other regions.

Area of Science:

  • Veterinary Microbiology
  • Bacterial Typing
  • Antimicrobial Resistance

Background:

  • Limited data exists on methicillin-resistant Staphylococcus pseudintermedius (MRSP) in Canadian dogs, particularly in Atlantic Canada.
  • Understanding MRSP strain types and resistance is crucial for canine health management.

Purpose of the Study:

  • To perform direct repeat unit (dru) typing on MRSP isolates from dogs in Atlantic Canada.
  • To characterize the antimicrobial resistance profiles of these MRSP isolates.
  • To compare MRSP strain distribution within Atlantic Canada and with other Canadian regions.

Main Methods:

  • Direct repeat unit (dru) typing was performed on 94 MRSP isolates from dogs (2010-2012).
  • Cluster analysis and antimicrobial susceptibility testing were conducted.
  • Statistical analysis was used to compare strain distribution across provinces.

Main Results:

  • The predominant MRSP dru types were dt11a (30.9%), dt10h (24.5%), dt9a (18.1%), and dt11af (10.6%).
  • A significant difference in dru type distribution was observed among Atlantic provinces (P = 0.01).
  • MRSP isolates exhibited lower resistance to non-β-lactam antimicrobials compared to previous reports.

Conclusions:

  • Atlantic Canada has a distinct distribution of MRSP dru types, with cluster 9a being less common.
  • Further research into MRSP dru types is needed to understand its Canadian epidemiology.
  • Characterization aids in managing MRSP infections in dogs.

More Related Videos

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus09:03

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

Published on: September 5, 2013

12.4K
Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes08:58

Isolation and Identification of Waterborne Antibiotic-Resistant Bacteria and Molecular Characterization of their Antibiotic Resistance Genes

Published on: March 3, 2023

8.2K

Related Concept Videos

Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...